大白菜高频再生体系的建立及策略

Strategies in Establishing Effective Regeneration System of Chinese Cabbage

就北方大白菜14个基因型进行组织培养再生比较,以筛选出的较高再生率的基因型为试材,在含不同配比的6-BA、TDZ、NAA及AgNO3、GA3、ABA的培养基中进行再生比较,分别建立了含有6-BA和TDZ的大白菜组织培养再生最佳培养基:MS+6-BA 5 mg/L+NAA 0.5 mg/L+AgNO32 mg/L和MS+TDZ0.1 mg/L+6-BA 5 mg/L+NAA 0.5 mg/L+AgNO32 mg/L+ABA 0.25 mg/L,大白菜再生频率最高可以达到90.6%,建立了大白菜高频再生体系,讨论分析了影响大白菜再生的主导因素并提出建立大白菜高频再生体系的可行策略。

This study evaluated shoot organogenic potential of 14 genotypes of Chinese cabbage （Brassica campestris L. ssp. pekinensis） , the regeneration frequency varied significantly. The genotype which had maximum shoot regeneration was chosen for investigating the optimum concentration of 6-BA or TDZ on shoot regeneration. The maximum frequency of shoot regeneration was obtained respectively in the mediums： MS ＋ 6-BA 5 mg/L ＋ NAA 0. 5 mg/L ＋ AgNO3 2 mg/L and MS ＋ TDZ 0. 1 mg/L ＋ 6-BA 5 mg/L ＋ NAA 0. 5 mg/L ＋ AgNO3 2 mg/L ＋ ABA 0.25 mg/L, and the highest frequency of shoot regeneration （90. 6% ） was achieved on the latter medium. In this research, The determine factors influencing shoot regeneration and strategies to establish efficient regeneration system of Chinese cabbage are discussed.