蝴蝶兰组织培养中的褐化控制研究

Studies of Antibrowning in the Tissue Culture of Phalaenopsis

系统研究了吸附剂(活性炭、PVP)与柠檬酸、半胱氨酸、谷胱甘肽、维生素C和硫代硫酸钠5种抗氧化剂对蝴蝶兰组培中褐化的影响。结果表明:活性炭能有效控制褐化和促进生长,但不利于分化;谷胱甘肽控制褐化的效果虽不及活性炭,但综合效果最佳。

In order to control the browning of tissue culture on Phalaenopsis, the contrast test of adsorbent： activated charcoal, PVP, and five antioxidants： citric acid, cysteine, glutathione, vitamin C, sodium thiosulfate in tissue culture of Phalaenopsis were studied. The results showed that activated charcoal can control browning and promote growth. But at the same time, it was adverse to the differentiation. Although the glutathione, which the effect of antibmwning was not as good as activated charcoal＇s, its resultant effect was the best one.