骨髓微转移在胃癌中的意义及其与VEGF关系的初步探讨

Preliminary Studies on Clinical Significance of the Micrometastases in Bone Marrow and the Relationship between VEGF and Micrometastasis in Gastric Cancer

目的:探讨骨髓微转移在胃癌中的意义,探讨骨髓微转移与VEGF之间的关系。方法:采用免疫组化SABC方法检测骨髓中的肿瘤细胞,ELISA方法检测血清VEGF表达。结果:骨髓微转移阳性胃癌患者较阴性患者肿瘤浸润深,腹腔播散及肝脏转移发生率高(P<0.05)。骨髓微转移阳性和阴性患者VEGF阳性率分别为77.8%(21/27),43.4%(23/53)(P<0.05)。结论:骨髓微转移与胃癌临床生物学行为关系密切,与VEGF表达水平相关,对胃癌的治疗和预后有着指导性的意义。

Objective： To investigate the clinical significance of the micrometastases in bone marrow in patients with gastric cancer and to clarify the relationship between VEGF and micrometastasis in gastric cancer. Methods： Micrometastases in bone marrow was tested in 80 patients with primary gastric cancer using an immunocytochemical assay. Also, vascular endothelial growth factor （VEGF） expression was examined by ELISA. Results： The micromatastases in bone marrow were related to depth of invasion, peritoneal dissemination and liver metastasis. Patients with positive micrometastasis in bone marrow had a higher VEGF positive rate （77.8%, 21/27） compared to negative micrometastasis patients （43.4%, 23/53）. Conclusion： The micrometastases in bone marrow are closely related to clinicopathological factors and VEGF in patients with gastric cancer, thus playing a key role in prediction for the prognosis of gastric cancer.