大蒜素与细胞周期特异性化疗药联合应用抗肿瘤的实验研究

The Studies on the Combined Effect of Allicin with Cell Cycles Specific Chemotherapeutic Drugs on Cancer Cells

目的:探讨大蒜素对人胃癌细胞株BGC-823、SGC7901生长的影响及其与细胞周期特异性化疗药联合应用抗肿瘤作用。方法:MTT法测定细胞增殖抑制率并测定药物半数抑制率(IC50);流式细胞仪检测细胞周期的改变,大蒜素与NVB、5-FU、MMC、PDD四种化疗药联合应用,观察细胞毒活性的改变。结果:大蒜素对BGC-823和SGC-7901两种细胞的生长均有明显的抑制作用,72hIC50分别为:30μg/ml和20μg/ml。以72hIC50浓度大蒜素分别作用于两种胃癌细胞株24h、48h后,流式细胞仪检测与对照组比G0/G1期细胞减少,G2/M期细胞明显增加,提示两种胃癌细胞株经大蒜素处理后,细胞周期阻滞于G2/M期。大蒜素与四种化疗药联合应用,结果发现作用于BGC-823细胞,NVB72h的IC50值由原来单独应用时的9.0μg/ml降至2.25μg/ml。作用于SGC-7901细胞,NVB72h的IC50值由原来单独应用时的43.0μg/ml降至12.5μg/ml,显示大蒜素与作用于G2/M期的细胞周期特异性化疗药NVB联合应用时可增强其对肿瘤细胞的杀伤作用。结论:大蒜素可使BGC-823和SGC-7901两种细胞的增殖受到抑制,细胞周期被阻滞在G2/M期。大蒜素与G2/M期特异性化疗药联合应用,可能具有协同抗肿瘤作用。

Objective： To study the effect of allicin on human gastric ceils BGC- 823 and SGC- 7901 and the combined effect of allicin with chemotherapeutic drugs to these cancer cells. Methods： To evaluate the inhibition rates of proliferation of cells and the IC50 of 72 hours of these cells by MTT, to analyze the change of cell cycle using flow cytometry, to study the change of cytotoxicity of NVB, 5-FU, MMC and PDD after combination of allicin with these chemotherapeutic drugs. Results： The cell growth of BGC-823 and SGC-7901 was inhibited by allicin and the 72-hour IC50 of allicin was 30ug/ ml and 20ug/ml respectively. After these cells were treated by allicin at the concentrations of 30ug/ml and 20ug/ml for 24 and 48 h respectively, the percentage of G0/G1 phase of the cells was decreased and that of G2/M phase cells was increased significantly in the allicin treated group, compared to the control group. It was shown that allicin caused arrest of gastric cancer cells in G2/M phase. In comparison with IC50 of NVB, 5-FU, MMC and PDD alone or combined with alliein, the 72-hour IC50 value of NVB in BGC-823 cells decreased from 9ug/ml to 2.25ug/ml, and that of 5-FU, MMC and PDD decreased from 6.25ug/ml, 12ug/ml and 0.07ug/ml to 5.8ug/ml, 9.8ug/ml and 0.15ug/ml （P〉0.05）, respectively; then the 72-hour IC50 value of NVB in SGC-7901 cells decreased from 43ug/ml to 12.5ug/ml, and that of 5-FU, MMC and PDD decreased from 1.5ug/ml, 0.38ug/ml and 0.56ug/ml to 2.0ug/ml,0.38ug/ml and 1.0ug/ml （P〉0.05）, respectively. Conclusions： Allicin has an inhibitory effect on gastric cells BGC-823 and SGC-7901. It can arrest gastric cancer cells in Ga/M phase and may enhance cytotoxicity of cell cycles specific chemotherapeutic drugs on the cancer cells.