神经节苷脂GM_1对应激性高血压大鼠MCAO后脑组织内Na~＋K~＋－ATPase活性变化的影响

Effect of GM_1 on the changes of Na￣+ K￣+ -ATPase activity following MCAO model brain ischemia in the stress hypertensive rat

采用ＳＤ大鼠，通过应激的方法制备高血压模型，并在此基础上阻断一侧大脑中动脉（ＭＣＡＯ），造成局灶性脑缺血。观察了缺血后３、１２、２４ｈ神经细胞膜Ｎａ＋Ｋ＋—ＡＴＰａｓｅ活性的变化，同时观察了神经节苷脂ＧＭ１对其活性的影响。结果发现，随缺血时间的延长酶活性逐渐降低，ＧＭ１术前３０ｍｉｎ给药（１０ｍｇ／ｋｇ）能明显抑制缺血后３ｈ酶活性的下降，但对缺血后１２、２４ｈ酶活性的下降无改善作用。提示ＧＭ１术前给药对缺血早期具有脑保护作用。

Changes in the activity of Na+K+ -ATPase and GM1 effects were studied on the stress induced hypertensive rat et 3,12,and 24hours following occlusion of middle cerebral artery(MCAO).The decreases in the enzyme activity was observed in the three grops.In the GM1 group,GM1(10mg/kg)was intraperitoneally injected 30 min. before occlusion,the decteases of Na+K+ -ATpase activity was signficantly attenuated in 3h group.It is suggested that the GM1 may protect function of cerebrum during the early phases of ischemic injury.