小檗碱对大鼠海马CA_1区迟发性神经元坏死的影响

The effect of berberine on delayed neuronal death in CA_1 in rat exposed to cerebral ischemia

本文采用Ｐｕｌｓｉｎｅｌｌｉ－Ｂｒｉｅｒｌｅｙ４血管结扎致ＳＤ大鼠全脑缺血（１０ｍｉｎ）再灌流模型，分别观察了早期不同再灌流时间（１２、２４、４８ｈ）点上，大鼠海马ＣＡ１区神经元的超微结构以及再灌７ｄ时光镜结构变化，同时观察了小檗碱对ＣＡ１区迟发性神经元坏死的影响。结果显示脑缺血再灌流早期，ＣＡ１区神经元超微结构发生明显改变，７ｄ时光镜下绝大部分细胞脱失；而用药组大鼠海马ＣＡ１区神经元在相应时间点上超微结构变化相对较轻，７ｄ时仍有绝大多数（８２％）细胞存活，细胞密度为１７２±１２．２个／ｍｍ，显著高于缺血对照组２７±７．６个／ｍｍ，Ｐ＜０．００１。提示小檗碱对大鼠短暂脑缺血再灌流造成的海马ＣＡ１区迟发性神经元坏死具有显著的对抗作用。

The ultrastructural changes in the pyramidal neurons of the CA1 sector in hippocampus were studied after 12hours,24hours,48hours.reperfusion,also light structural changes after 7day.following a transient 10 min.cerebral ischemia induced by 4 vessel occlusion described by Pulsinelli-Brierly previously.The effect of berberine on delayed neuronal death in this model was observed simultaneously.The results showed that, at early reperfusion stages,the ultrastructural changes were apparent.After 7d,almost all neurons in CA1 were disappear,with the density 27±7.6/mm.But in berberine group,the subcellular changes were moderate,and after 7d reperfusim,most cells were intact,with the density of CA1 pyramidal neuron 172±12.2/mm.It is suggested that berberine effectirely protects neurons in CA1 from DND induced by transient cerebral ischemia followed by reperfusion.