摘要
普通冬小麦品系99-2439在郑州连续4年对田间白粉菌(Blumeriagraminissp.tritici)表现高抗,但其抗性基因来源不清。通过染色体C-分带和1RS染色体特异性SCAR标记鉴定,表明它是一个小麦-黑麦(Triticumaestivum-Secalecereale)1BL/1RS异易位系。通过对中国春×99-2439杂交F2代分离群体抗性鉴定和1RS染色体臂检测结果分析,证明该抗病基因不在1RS染色体臂上。用单孢小麦白粉菌分离株对其抗性遗传进行研究,结果表明,99-2439的白粉病抗性由一对小种专化、隐性抗病基因控制。由于携带Pm5a的Hope/8Cc对中国的21个小麦白粉菌分离菌株均高度感病,而99-2439高抗混和白粉菌和5个单孢分离菌株,所以,99-2439所携带的抗白粉病基因不同于Pm5a。
Common winter wheat （Triticum aestivum） line 99-2439 has shown high resistance to Blumeria graminis sp. tritici in the field in Zhengzhou for 4 years. However, the origin of its powdery mildew resistance genes was unclear. We identified 99-2439 as a Triticum aestivumSecale cereale 1BL/1RS alien translocation line by chromosome C-banding and 1RS-specific SCAR marker detection. Powdery mildew evaluation and 1RS detection of an F2 segregation population derived from the cross between Chinese Spring wheat and line 99-2439 demonstrated that the powdery mildew resistance gene was located on the 1RS chromosome arm. Inheritance study using a single spore isolate of B. graminis showed that line 99-2439 harbored a racespecific recessive powdery mildew resistance gene. Because 99-2439 was highly resistant to mixed fungi and 5 single spore isolates ofB. graminis, the powdery mildew resistance gene of 99- 2439 was different from Pm5a of Hope/8Cc, which was highly susceptible to all 21 isolates of B. graminis in China.
出处
《植物学通报》
CSCD
北大核心
2005年第4期439-444,共6页
Chinese Bulletin of Botany
基金
河南省杰出青年科学基金(0212000800)
河南省科技厅重点攻关项目(123014400)资助。
