热疗对荷VX_2瘤动物细胞因子影响的实验研究

Effect of hyperthermia on VX_2 carcinoma bearing rabbits

目的探讨热疗对荷VX2瘤动物细胞因子的影响。方法选健康雄性大白兔40只,随机分为热疗组20只、对照组20只。移植VX2瘤株11d后,对实验组动物进行热疗照射,监测白介素-2(IL-2)、肿瘤坏死因子(TNF)、胰岛素样生长因子(IGF)的变化。结果实验动物生存期较对照组延长15d左右。移植前IL-2、TNF、IGF为(134±6)ng/ml、(4·5±0·9)ng/ml、(96±28)ng/ml;治疗后1周为(81±7)ng/ml、(7·6±1·8)ng/ml、(678±183)ng/ml;3周为(61·8±2·0)ng/ml、(15·1±3·0)ng/ml、(991±108)ng/ml;4周为(54·8±2·6)ng/ml、(17·2±2·1)ng/ml、(1136±89)ng/ml。结论热疗对于表浅肿瘤的治疗是有效的辅助疗法;热疗后肿瘤细胞变性坏死的分解产物被机体吸收后作为一种抗原,刺激机体的免疫系统产生细胞因子,起到抗肿瘤免疫功能。

Objective To study the effect of hyperthermia on cytokines of VX2 carcinoma bearing rabbits. Method Forty healthy male rabbits were randomly divided into heat therapy group and control group. Rabbits in heat therapy group underwent hyperthermia 11 days after inoculated with VX2 cells. IL-2, tumor necrosis factor （TNF） and insulinlike growth factor （IGF） were measured before and after heat therapy. Results In heat therapy group survival was prolonged for 15 days. IL-2 decreased gradually from （ 134 ± 6） ng/ml before heat therapy, （ 81±7 ） ng/ml one week after, （61.8 v 2. 0） ng/ml at 3 weeks, and （54. 8±2.6） ng/ml at 4 weeks along with an increase of TNF and IGF （4. 5±0. 9） ng/ml, and （96 ±28） ng/ml; （7.6± 1.8） ng/ml and （678±183） ng,/ml; （15.1±3.0） ng,/ml and （991 ±108） ng/ml; （ 17. 2 ± 2. 1 ） ng/ml and （ 1136 ± 89 ） ng/ml respectively at the corresponding time period. Conclusion Heat therapy is effective for the treatment of superficial tumors probably through a mechanism of antigenantibody reaction.