灵芝虫草菌丝体对大鼠NK细胞活性影响的流式细胞术研究

FCM STUDY ABOUT EFFECTS OF GANODERMALUCIDUM AND CORDYCEPICMYCELIA ON ACTIVITY OF NK CELL IN RATS.

目的:研究灵芝与虫草菌丝体联合使用对SD大鼠NK细胞免疫调节的作用。方法:将灵芝虫草菌丝按0.75%和1.50%的比例掺入饲料中(分别相当于0.75 g/kg、1.50 g/kg BW剂量)给SD大鼠自由摄食,连续30 d后取其外周血用流式细胞术检测NK细胞活性。结果:1.50g/kg BW剂量组大鼠外周血CDl61a-/CD25+和CDl61a+/CD25+的表达明显高于对照组,差异比较有统计学意义(P<0.05)。结论:灵芝虫草菌丝体有增强大鼠NK细胞活性的作用。

Objective： To learn the effects of Ganodermalucidum and Cordycepicmycelia on NK cellular immunity of rats. Methods： 0.75 and 1.50 percent of Ganodermalucidum and Cordycepicmycelia were mixed into feeds according as the 0.75, 1.50g/kg BW doses.Tile mixed feeds were given to SD rats and thirty days later, the blood of rats was collected to test the activity of NK ceils with FCM. Results： Compared with the negative control group, peripheral blood NK cell sub-cluster CD161a^-/CD25^＋ and CD161a^＋/CD25^＋ of 1.50g/kg BW were both significantly increased （P〈0.05） . Conclusion： Ganodermalucidum and Cordycepicmycelia have enhancing effects on the activity of NK cell of rats.