人胰岛素转基因小鼠模型的建立

Construction of animal model of transgenic mice expressing human insulin

目的:建立人胰岛素的转基因小鼠模型。方法:通过原核显微注射法,把外源基因pEF1α-m INS注射入FVB种小鼠受精卵,胚胎移植给同期发情的假孕受体母鼠获得子代小鼠。结果:移植注射胚胎204枚给38只假孕小鼠共出生108只后代鼠,经PCR和Southern b lot检测到5只阳性小鼠。结论:通过显微注射法使外源基因pEF1-αm INS在小鼠基因组中得到整合,建立了人胰岛素的转基因小鼠模型。

Objective： To construct transgenic mice model expressing human insulin. Methods： pEF1α-mINS fragments were microinjected into fertilized mice eggs. Those eggs were transplanted into the oviducts of the pseudopregnant mice. Results：Transgenic FVB mice were obtained by microinjection. PCR and Southern blot analysis showed 5 of 108 mice were transgenic. Conclusion：pEF1α-mINS fragment can integrate into mice genomic DNA. An animal model of transgenic mice expressing human insulin can be successfully established.