高压氧对急性一氧化碳中毒小鼠脑组织细胞因子的影响

Effects of hyperbaric oxygen on the levels of cytokines in mice with acute carbon monoxide poisoning

目的:观察高压氧(HBO)对急性一氧化碳(CO)中毒小鼠脑组织白细胞介素1β(IL-1β)、肿瘤坏死因子γ(TNF-γ)、白细胞介素4(IL-4)及白细胞介素10(IL-10)的水平影响。方法:建立CO中毒模型,常压氧(NBO)、高压氧干预治疗。ELISA法检测各组小鼠脑组织细胞因子的浓度。结果:CO中毒组IL-1β和TNF-α含量明显高于对照组(P<0.01),而IL-4和IL-10则明显低于对照组(P<0.01)。HBO治疗组与CO中毒组比较,IL-1β和TNF-α含量显著下降(P<0.01),而IL-4和IL-10含量显著升高(P<0.01)。NBO治疗组IL-1β和TNF-α低于CO中毒组,而IL-4和IL-10高于CO中毒组,但差异不显著。结论:HBO可对抗CO中毒小鼠脑组织的毒性作用。

Objective： To observe the effects of hyperbaric oxygen on the levels of interleukin-1β （IL-1β）, tumor necrosis factor-α（TNF-α） , interleukin-4 （IL-4）, and interleukin-10 （IL-10） in mice with acute carbon monoxide（CO） poisoning. Methods：We established animal models of CO poisoning and treated the animals with normobarie oxygen and hyperbaric oxygen. The levels of eytokines in brain tissues were determined by using ELISA method. Results：The levels of IL-1β and TNF-α in CO poisoning group were higher than those in control group（ P 〈 0. 01.）. The levels of IL-4 and IL-10 in CO poisoning group were lower than those in control group（ P 〈 0.01 ）. In hyperbaric oxygen group, levels of IL-1β and TNF-α decreased significantly （P 〈 0.01 ） ,and levels of IL-4 and IL-10 increased significantly （ P 〈 0.01 ）. There was no significant difference between normobarie oxygen group and CO poisoning group（ P 〉 0.05 ）. Conclusion：Hyperbaric oxygen can antagonize brain damage caused by CO poisoning in mice.