高效液相色谱法测定头孢克罗血浆浓度及其药动学研究

Determination of cefaclor in Plasma by HPLC and its pharmacokinetics

本文用离子对高效液相色谱法（ＨＰＬＣ）测定血浆中的头孢克罗（Ｃｅｆａｃｌｏｒ）浓度。血浆样品用１％磷酸甲醇溶液提取。色谱条件为：紫外检测波长λ２６５ｎｍ；分析柱ＵｌｔｒａｓｐｈｅｒｅＴＭ“ＩＰ”２５０ｍｍ×４．６ｍｍ；流动相甲醇：四氢呋喃：水（每升水中含１．０ｇ庚磺酸钠，１１ｇ三乙胺，２０．５ｇ磷酸）。本法最小检测限５０ｎｇ／ｍｌ。本法对１０名受试者口服２５０ｍｇ头孢克罗后进行药动学和相对生物利用度研究。头孢克罗为一房室模型，其Ｔ１／２（ｋｅ）０．６ｈ，Ｔｍａｘ０．４～０．７ｈ，Ｃｍａｘ４．９２～６．５０μｇ／ｍｌ。颗粒剂的相对生物利用度为１．０７±０．１３。

efaclor is a new cephalosporin antibiotic for oral use. Cefaclor in plasma was deterrnined hy IP-HPLC. Theplasma sample was extracted with a mixture solution of phosphoric acidand methanol. The mobile phase wasmethanol :tetrahydrofuran :water (contain 1.0g heptanesulfonate sodium , 11.0g triethylamine and 20. sg phospho-ric in 1. 0L water). Analrtical column was Ultrasphere TM'IP''(250mm × 4. 6mm ID). The detection wavelengthwas 265nm. The minimun detective concentration was 50 ng/ml. Using this method, pharmacokinetics andbioavailaility of cefaclor in ten male healthy volunteers after oral administration of 250mg dosage was studied. Theplasma-time concentration curve of volunteers was one-compartment model. The half-life time of elimination was0. 6h,Tmax 0. 4h￣ 0. 7h and Cmax .4. 92and 6. 50μg/ml. The relative bioavailability of cefaclor granule was 1. 07 ±0. 13.