摘要
采用新菌株Bacillus subtilisJSIM-518,在22 L自控发酵罐上进行不同补料方式和所补原料的发酵研究.酵母粉连续补料,对鸟苷积累不甚明显,应用指数函数补料方式,匀速补入RNA和腺嘌呤物质,添加速率K值显得非常关键,当K=0.16时积累鸟苷最高,积累质量浓度为34.14g/L,高于和低于此值时,鸟苷的积累量都呈递减的趋势.
With the stain of guanosine producer, Bacillus subtilis JSIM-518, the feeding conditions was studied on a 22-liter auto-fermentor by using various supplement nutrients and feeding ways. The guanosine production did not change by the continuous feeding yeast powder. By the exponentially feeding with RNA or adenine, the highest concentration of guanosine reached 34.14 g/L when setting an optimal constant K at 0.16. When a higher or lower K was selceted, the guanosine concentration decreased.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2005年第5期21-24,共4页
Journal of Food Science and Biotechnology
基金
国家"十五"科技攻关项目(2004BA713B05-09)资助课题
关键词
鸟苷
发酵
函数补料
连续补料
guanosine
fermentation
function feeding
continuous feeding