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大肠杆菌产生的聚唾液酸的结构 被引量：3

Structure Analysis of Polysialic Acid in Escherichia coli
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摘要 研究了大肠杆菌产生的聚唾液酸的分子结构,对聚唾液酸的分子基团特征进行了红外光谱分析,研究了聚唾液酸糖苷键的连接方式和构型.发酵液中的聚唾液酸经超滤浓缩、有机沉淀和离子交换层析处理,得到高纯度的聚唾液酸.经13C-NMR分析和比较,表明所得到的聚唾液酸是一种-α2,8糖苷键连接的同聚物. The structure of polysialic acid was investigated with infrared spectra and ^13C-NMR methods. The polysialic acid was purified from Escherichia coli broth with ultrafiltration, organic solvent precipitation and ion-exchange chromatography. The group characteristics of the polyialic acid were confirmed with infrared spectra and identified with that of standard sample. The polysialic acid in E. coli K-235 was identified as α-2,8 glycosidic linkage homopolymer of Nacetyl-neuraminic acid.
作者 郑志永 詹晓北 吴剑荣 朱莉
机构地区 江南大学工业生物技术教育部重点实验室
出处 《食品与生物技术学报》 CAS CSCD 北大核心 2005年第5期38-41,共4页 Journal of Food Science and Biotechnology
基金 江苏省自然科学基金项目(BK99190)资助课题
关键词 聚唾液酸 ^13C-NMR 红外光谱 大肠杆菌 polysialic acid ^13C-NMR infrared spectra Escherichia coli
分类号 Q936 [生物学—微生物学]
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参考文献10

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共引文献21

同被引文献38

  • 1孔庆胜,迮玉官.聚乙二醇修饰超氧化物歧化酶的基础研究[J].济宁医学院学报,2006,29(1):16-17. 被引量：8
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食品与生物技术学报
2005年 第5期

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