旋磁场防治大鼠缺血再灌注脑损伤

Reperfusion injury of acute cerebral ischemia in Wistar rats by treatment of rotating magnetic field

用四血管阻断法造成大鼠全脑缺血，缺血半小时后恢复双侧颈总动脉血流半小时，此时大鼠脑组织和血浆ＬＰＯ含量显著增高、脑组织ＳＯＤ活性和ＶｉｔＥ含量明显降低（Ｐ＜０．０５），大脑皮层神经细胞超微结构和血脑屏障明显受损。如在大鼠脑缺血半小时再灌流开始时，用旋磁场（０．０８Ｔ，２５００ｒ／ｍｉｎ）作用于双侧颈动脉区２０ｍｉｎ，大鼠脑组织和血浆ＬＰＯ含量降低、脑组织ＳＯＤ活性增高（Ｐ＜０．０５），但脑组织ＶｉｔＥ含量无明显变化（Ｐ＞０．０５），而大脑皮层神经细胞超微结构和血脑屏障损伤减轻。

The complete cerebral ischemia(CCI)models were made with 4 vessels occlusion in awake Wistar rats. Recirculation in bilateral carotids for 30min after 30min CCI in rats,brain tissues and plasma lipid peroxidation(LPO) values increased(P<0.05),superoxide dismutase(SOD) activities and vitamin E(VitE ) values in brain tissues decreased significantly (P< 0. 05 ),and the cortex neuronal ultrastructures and blood一brain barriers (BBB) were damaged severely. At the beginning of brain tissues reperfusion after 30min CCI in Wistar rats,rotating magnetic field(RMF,0.08T, 2500rpm)were used by placing it on bilateral carotic areas for 20min. The effects of RMF were decreasing LPO values in brain tissues and plasma(P<0. 05),increasing brain tissues SOD activities(P<0. 05)yet had no significant effects on brain tissue;VitE values(P>0. 05). Author's address Guangdong Medical College Affilated Hospital,524001