苹果酸、γ-氨基丁酸对Hela细胞端粒酶活性的影响

Effect of Malate and Gamma-aminobutyric Acid on Telomerase Activity of Hela Cells

目的:观察苹果酸、γ-氨基丁酸(GABA)对Hela细胞端粒酶活性的影响。方法:苹果酸、γ-氨基丁酸作用于Hela细胞不同时间后,用TRAP-银染半定量法检测端粒酶活性的变化。结果:1.将不同浓度的苹果酸作用于Hela细胞48 h后,端粒酶活性降低,以10μmol/L浓度时作用活性最低。10μmol/L苹果酸作用于Hela细胞不同时间,端粒酶活性于作用8 h时就有所下降,当作用48 h时与对照组相比下降最多达到11%。2.将不同浓度的GABA作用于Hela细胞48 h后,端粒酶活性降低,以1 mmol/L浓度时作用活性最低。1 mmol/L GABA作用于Hela细胞不同时间,结果显示端粒酶活性于作用8 h时就有所下降,当作用48 h时与对照组相比几乎下降最多达到13%。结论:苹果酸,GABA对Hela细胞端粒酶活性起下调作用。

Objective：To observe effect of malate and gamma-aminobutyric（GABA） on telomerase activity of Hela cells, Methods：Hela cells were treated with various concentration of malate and GABA respectively for different time and then the activity of telomerase were examined by the method of TRAP, Results： （ 1 ） After treatment with malate at various concentration for 48 h, malate decreased telomerase activity, The maximum down-regulation was obtained at a concentration of 10μmol/L, A time course experiment of telomerase activity in Hela cells treated with 10 μmol/L malate revealed decreased level as early as 8 h after treatment beginning and down to approximately 11% as contrast to the control level at 48 h. （2） After treatment with GABA at various concentration for 48 h, GABA decreased telomerase activity, The maximum down-regulation was obtained at a concentration of 1 mmol/L, A time course experiment of telomerase activity in Hela cells treated with 1 mmol/L GABA revealed decreased level as early as 8 h after treatment beginning and down to approximately 13% as contrast to the control level at 48 h. Conclusion：The telomerase activity of Hela cells can be down-regulated by malate and GABA,