摘要
以天津短杆菌(Brevibacteriumtianjincese)T_(6~13)为出发菌株,采用紫外线连续诱变处理,获得一株抗磺胺胍和利福平的L-异亮氨酸营养缺陷型SF28菌株,该菌株摇瓶发酵产L-脯氨酸28.5g/L,经过培养基成分考查,该菌株在32℃培养72h可产L-脯氨酸37.8g/L。
An L-isoleucine auxotropy mutant,strain SF28,resistant to sulfaguanidine and rifampin was obtained from Brevibacterium Tianjinese T6~13 by serial treatment with ultraviolet ray.This mutant produces L-proline in shaking flask at 32℃ when fermented for 72 hours,and it can produce 37.8g/L of L-proline on examination of medium composition.
出处
《沈阳药科大学学报》
CAS
CSCD
1996年第2期112-116,共5页
Journal of Shenyang Pharmaceutical University
基金
青年科技发展基金