一株L－脯氨酸产生菌的选育

REDING OF AN L-PROLINE PRODUCER

以天津短杆菌（Ｂｒｅｖｉｂａｃｔｅｒｉｕｍｔｉａｎｊｉｎｃｅｓｅ）Ｔ_（６～１３）为出发菌株，采用紫外线连续诱变处理，获得一株抗磺胺胍和利福平的Ｌ－异亮氨酸营养缺陷型ＳＦ２８菌株，该菌株摇瓶发酵产Ｌ－脯氨酸２８．５ｇ／Ｌ，经过培养基成分考查，该菌株在３２℃培养７２ｈ可产Ｌ－脯氨酸３７．８ｇ／Ｌ。

An L-isoleucine auxotropy mutant,strain SF28,resistant to sulfaguanidine and rifampin was obtained from Brevibacterium Tianjinese T6～13 by serial treatment with ultraviolet ray.This mutant produces L-proline in shaking flask at 32℃ when fermented for 72 hours,and it can produce 37.8g/L of L-proline on examination of medium composition.