茶多酚单体和黄芩苷对紫外线辐射皮肤成纤维细胞的影响

Photoprotective effects of tea polyphenol monomer and baicalin in fibroblasts from ultraviolet radiation

目的:研究中药活性成分茶多酚单体没食子儿茶素没食子酸酯(EGCG)、黄芩苷对紫外线辐射损伤的成纤维细胞形态、增殖活性及产生IL-6、TNF-α的影响,探讨其光保护作用。方法:采用30mJ/cm2、60mJ/cm2、90mJ/cm2剂量UVB照射体外培养的原代人皮肤成纤维细胞,以中药单体EGCG和黄芩苷进行干预处理,倒置相差显微镜观察细胞受损程度,以甲唑盐比色试验(MTT法)检测细胞增殖活性,酶联免疫吸附试验(ELISA)检测上清液TNF-α、IL-6分泌量。结果:UVB照射引起皮肤成纤维细胞形态受损,其损伤程度呈剂量依赖性,细胞增殖活性下降28%～44%,其下降程度与照射剂量成正相关(P<0.05),UVB照射后细胞因子分泌增加,加入中药单体处理后细胞活性有不同程度恢复(4%～22%),IL-6、TNF-α分泌量显著下降,差异均有统计学意义。结论:EGCG、黄芩苷具有光保护性能,抑制炎症细胞因子IL-6、TNF-α分泌可能是其减轻紫外线辐射损伤的机制之一。

Objective To investigate the effects of epigallocatechin gallate （EGCG） and baicalin on human skin fibroblasts from ultraviolet radiation. Methods Fibroblasts were obtained and cultured from human skin.The cells were shammed or irradiated with different dosages of UVB radiation and treated with the above TCM monomers. The damage of fibroblasts was observed with inverted phase contrast microscope.Cellular proliferation activity was detected by MTT method.The secretory volume of IL-6 and TNF-α was measured by ELISE. Results The damage degree of the cells irradiated was dependent with the irradiated doses and the proliferation activity reduced by 28%-44% .The secretory volume of cytokine was increased with UVB exposure.The intervention of the above drugs could recover the cellular growth activity by 4%-22%,the cytokine secretion amount decreased .The difference among them was significant. Conclusion The above results indicate that EGCG and baicalin both have photoprotectlve effection against UVB radiation.Inhibition of cytokine secretion may be the one of mechanisms to reduce the damage from UVB irradiation.