异氟醚减少离体鼠肝缺氧-复氧损伤

Isoflurane decreases anoxia-reoxygenation injury in isolated rat liver

目的研究不同浓度异氟醚对离体鼠肝缺氧-复氧损伤的影响。方法将大鼠肝脏取下,置于离体鼠肝灌流仪中,以经95%O2/5%CO2饱和的改良克-林氏碳酸氢盐缓冲液恒压灌流(有氧状态用1.2kPa,缺氧状态用0.2kPa),维持生理pH值和温度,实时监测灌流液中乳酸脱氢酶(LDH)变化。不同浓度异氟醚随混合气带入人工肺。用细胞色素C还原法测非循环灌流中氧自由基(O2-)产量。结果(1)异氟醚对基础状态下离体肝LDH释放无明显影响,而对缺氧-复氧后离体肝LDH释放呈剂量依赖性抑制作用。(2)2 MAC异氟醚可明显抑制肝复氧后O2-产生。结论异氟醚抑制了复氧后LDH升高与抑制复氧即刻O2-产生密切相关,异氟醚可能通过减少细胞外氧应激,从而保护肝脏免受损伤。

Objective To investigate the protective effects of isoflurane on anoxia-reoxygenation injury in isolated rat liver. Methods Rat livers were harvested and perfused with modified Krebs＇ buffer saturated with 95% O2/5% CO2 and added with 10 mM glucose and 1% albumin. The perfusion pressures were controlled constantly at 1.2kPa（oxygenation） or 0.2kPa （anoxia）. pH and temperature were maintained properly, and oxygen pressure and liver flow were measured. Different concentration of isoflurane was brought into artificial lung with mixed air. O2^- was measured by cytochrome C reduction technique. Results （1）Isoflurane did not change LDH release in isolated rat liver normally, while it effectively inhibited LDH release after anoxia and reoxygenation. （2）2 MAC isoflurane decreased O2^- release obviously after anoxia and reoxygenation in isolated rat liver. Conclusion Isoflurane can inhibit LDH by reducing O2^- release after anoxia and reoxygenation in isolated rat liver, and may protect hepatic function by inhibiting oxwen stress outside cells.