冷冻手术对正常鼠脑组织化学解剖结构及免疫功能的影响

Effect of Cerebral Local Cryosurgery on Chemical Neuroan atomical Features and Function of Immunity in Normal Mouse Brain

目的:通过检测反映脑组织损伤程度及免疫功能的实验指标客观评价冷冻手术与常规手术对脑组织的创伤程度。方法:实验小鼠随机分为正常组、冷冻手术组、常规手术组。以常规免疫组织化学及放射免疫法检测正常组、冷冻手术组及常规手术组术后相同时间段内的脑组织c-fos、NOS、CD4、CD8的表达程度及IL-2、IL-6脑组织含量,相同时间段内以t检验进行统计学处理,不同时间段内行Newman-Keuls检验。结果:相同时间段内冷冻手术组c-fos、nNOS的表达明显低于常规手术组,而CD4、CD8的表达明显高于常规手术组,统计学显示差异有显著性(P<0.01);冷冻手术组IL-2、IL-6的含量明显高于正常组及常规手术组,并形成两次分泌高峰,统计学显示结果差异有显著性(P<0.01)。结论:冷冻手术对脑组织的创伤程度明显小于常规手术,同时冷冻手术可以激发脑组织的免疫功能。

Objective： To evaluate the damage of the mouse＇s brain tissue treated by the cryosurgery and routing surgery. Methods： All mice were divided into 3 groups as the normal, cryosurgery and conventional surgery group. The expressions of c-los, nNOS, CD4 and CD8 in the cortex, hippocampus and brain stem were assessed, and levels of IL-2 and IL-6 in the brain were detected with radioimmunoassay. All data were analyzed with SPSS 10.0 software. Results： Tissue damages in distinct phases were observed, and the strong expression of c-los and nNOS existed in the conventional surgery groups, but significant increasing of CD4,CD8 and IL-2 and -6 were detected in the cryosurgery groups（P〈0.01）. Conclusion： Cryoinjury-induced tissue injury was markedly attenuated and in some certain of degree, cryosurgery could induce the reaction of the immune.