化学发光免疫分析法测定血清甲状腺素

MEASUREMENT OF SERUM THYROXINE BY CHEMILUMINESCENT IMMUNOASSAY

建立了以鲁米诺、过氧化氢作为发光底物，辣根过氧化物酶作为标记酶的化学发光免疫分析法，用于血清中甲状腺素水平测定，此法灵敏度为４．６ｎｇ／ｍｌ，不同水平Ｔ＿４批内变异系数为４．５％～７．８％，批间变异系数为７．０％～７．４％。加入Ｔ＿４５０ｎｇ／ｍｌ、１００ｎｇ／ｍｌ、２００ｎｇ／ｍｌ，回收率分别为１０５％、１０１％、１０４％，与放免法有较好的相关性，ｒ＝０．８３１，Ｐ＜０．００１，关系式为Ｔ４－ＣＬＩＡ＝０．９１５Ｔ４－ＲＩＡ＋２７．１（ｎｇ／ｍｌ），ｎ＝２６。 此法操作简便，检测速度快，适合于临床应用。

Established a method to detect serum thyroxine by chemiluminescent immunoassay in which luminol and hydrogen peroxide were used as luminescent substrates and horseradish peroxidase as label.It had a sensitivity of 4.6ng/ml.Within-run coefficient of variation was 4.5%～7.8%,and between-run coefficient of variation was 7.0%～7.4%at different thyroxine levels,Its recovery rates were 105%,101%,and 104%at concentrations of 50,100,200ng/ml.The linear correlation coefficient between the T4 values as determined by this method and radioimmunoassay was 0.831,T4-CLIA=0.915 T4-RIA-27.1(ng/ml),n=26.The method is applicable to clinical laboratory for it was easy and quick to do.