脆性三联组氨酸基因在胃癌中的表达及其临床意义

Fragile histidine triad expression and its clinical significance in gastric cancer

目的:探讨FHIT基因在胃癌中的表达及意义。方法:采用免疫组化法检测49例胃癌、21例正常胃黏膜、31例萎缩性胃炎、27例异形增生组织中FHIT基因表达,并分析与临床分期、分化程度、淋巴转移及3年生存期的关系。结果:FHIT基因在进展期胃癌中的阳性表达率为18.4%,早期胃癌中为27.3%(3/11),均低于异型增生(55.6%,15/27)、萎缩性胃炎(74.2%,24/31)及正常胃黏膜(100%,P=0.000)中的表达。FHIT基因在早期胃癌中的表达高于中、晚期胃癌(P=0.001);在高分化组中低于中、低分化组(P=0.022),无淋巴结转移者与淋巴结转移者表达无统计学差别(P=0.065)。随访的49例胃癌资料中,FHIT基因表达阴性者生存时间明显低于表达阳性者(Log rank=5.06,P=0.0245)。结论:FHIT基因的表达缺失与胃癌的发生、发展及预后有关,FHIT水平可作为判断胃癌预后的一种指标。

Objective To determine the fragile histidine traid （FHIT） expression and to analyze its clinical significance in gastric cancer. Methods FHIT expression was determined using immunohistochemistrv in 49 cases of gastric cancer, 21 cases of normal gastric nmeous membrane. 31 cases of atrophic gastritis, and 27 cases of dysplasia, and the correlation between the absence of FHIT expression and the clinical staging, grade, and survival time in patients with gastric cancer was studied. Results The positive expression rate of FHIT gene was 18.4%, 27.3%, 55.6%, 74.2% and 100% in tissues of advanced stage gastric cancer, early stage gastric caneer, dysplasia, atrophic gastritis and normal gastric mucous membrane, respectively. The absence of FHIT gene correlated with advanced staging （P=0.001） and high histologic grade of cancer （P=0.002）. Gastric cancer patients expressing FHIT gene died of their disease signifieantly later than those who did not express FHIT （Log rank=5.06. P=0.0245）. There was no significanet difference in FHIT expression between patients with positive node metastasis and those without（P=0.065）. Conclusions The absenee of FHIT gene correlates with the pathogenesis, progression and prognosis of gastric eancer. To examine FHIT by immunohistochemistry will be a useful indicator of prognosis.