2型糖尿病肾病患者血清晚期蛋白氧化产物的变化

Changes of serum advanced oxidation protein products in type 2 diabetes mellitus patients with diabetic nephropathy

目的:研究糖尿病肾病患者血清蛋白氧化指标晚期蛋白氧化产物(AOPP)的改变及其与超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)的关系。方法:2型糖尿病患者85例,分为无糖尿病肾病(DM)组(n=25)、早期糖尿病肾病(DN3)组(n=24)、临床糖尿病肾病(DN4)组(n=17)、终末期糖尿病肾病(DN5)组(n=19)。用改进后的Wikto-Sarsat方法分别测定各组的血清AOPP,黄嘌呤氧化酶法测定SOD,DTNB[5,5,-二硫代双(2-硝基苯甲酸)]显色法测定GPx。结果:DN5组患者的血清AOPP[(117.8±64.8)μmol/L]和DN4组的血清AOPP[(80.0±23.0)μmol/L]显著高于DM组[(58.2±17.7)μmol/L],P<0.01。DN3组血清AOPP[(72.7±17.2)μmol/L]与DM组间无显著差异。血清AOPP与肌酐水平呈显著正相关(r=0.277,P<0.05),与尿微量白蛋白无明显相关性(r=0.06,P>0.05)。血清AOPP与SOD及GPx呈显著负相关(r分别为-0.217和-0.374,P<0.05)。结论:糖尿病肾病患者的血清蛋白氧化较无糖尿病肾病患者显著增强。

Objective：To study the changes of serum advanced oxidation protein products（AOPP） in patients with diabetic nephropathy（DN） and its correlationship with superoxide dismutase（SOD） and glutathione peroxidase（GPx）. Methods： Eighty-five diabete mellitus （DM） patients were divided into 4 groups： non-DN group（DM, n=25）, early-stage DN group（DN3, n=24）, significant DN group（DN4, n=17） and end-stage DN group （DN5, n=17 ）. The concentration of serum AOPP was measured spectrophotometerically, serum SOD was determined by xanthine oxidase test and GPx by DTNB reaction test. Results： The concentrations of AOPP in patients of DN5 group （[117.8±64.8]μmol/L） and DN4 group （[80.0±23.0]μmol/L） were significantly higher than that in DM group （[58.2±17.7] μmol/L,P〈0.01）. There was no significant difference of AOPP concentrations between DN3 group （[72.7±17.2]μmol/L） and DM group. Serum AOPP was positively correlated with serum creatinine（r=0.277, P〉0.05） and negatively correlated with SOD and GPx（r=-0.217, r=-0.374, P〈0.05）. Conclusion： The protein oxidation in patients with DN is enhanced compared to those without DN.