摘要
目的:研究糖尿病肾病患者血清蛋白氧化指标晚期蛋白氧化产物(AOPP)的改变及其与超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)的关系。方法:2型糖尿病患者85例,分为无糖尿病肾病(DM)组(n=25)、早期糖尿病肾病(DN3)组(n=24)、临床糖尿病肾病(DN4)组(n=17)、终末期糖尿病肾病(DN5)组(n=19)。用改进后的Wikto-Sarsat方法分别测定各组的血清AOPP,黄嘌呤氧化酶法测定SOD,DTNB[5,5,-二硫代双(2-硝基苯甲酸)]显色法测定GPx。结果:DN5组患者的血清AOPP[(117.8±64.8)μmol/L]和DN4组的血清AOPP[(80.0±23.0)μmol/L]显著高于DM组[(58.2±17.7)μmol/L],P<0.01。DN3组血清AOPP[(72.7±17.2)μmol/L]与DM组间无显著差异。血清AOPP与肌酐水平呈显著正相关(r=0.277,P<0.05),与尿微量白蛋白无明显相关性(r=0.06,P>0.05)。血清AOPP与SOD及GPx呈显著负相关(r分别为-0.217和-0.374,P<0.05)。结论:糖尿病肾病患者的血清蛋白氧化较无糖尿病肾病患者显著增强。
Objective:To study the changes of serum advanced oxidation protein products(AOPP) in patients with diabetic nephropathy(DN) and its correlationship with superoxide dismutase(SOD) and glutathione peroxidase(GPx). Methods: Eighty-five diabete mellitus (DM) patients were divided into 4 groups: non-DN group(DM, n=25), early-stage DN group(DN3, n=24), significant DN group(DN4, n=17) and end-stage DN group (DN5, n=17 ). The concentration of serum AOPP was measured spectrophotometerically, serum SOD was determined by xanthine oxidase test and GPx by DTNB reaction test. Results: The concentrations of AOPP in patients of DN5 group ([117.8±64.8]μmol/L) and DN4 group ([80.0±23.0]μmol/L) were significantly higher than that in DM group ([58.2±17.7] μmol/L,P〈0.01). There was no significant difference of AOPP concentrations between DN3 group ([72.7±17.2]μmol/L) and DM group. Serum AOPP was positively correlated with serum creatinine(r=0.277, P〉0.05) and negatively correlated with SOD and GPx(r=-0.217, r=-0.374, P〈0.05). Conclusion: The protein oxidation in patients with DN is enhanced compared to those without DN.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第9期1037-1039,共3页
Academic Journal of Second Military Medical University
基金
广东省重点科技攻关项目(99m04b16g)