缺氧复合梭曼中毒对PC_(12)细胞中IL-4、IL-8和TPK活性的影响

Effect of Soman and hypoxia on TPK activity and cytokines in PC_(12) cell line

目的研究缺氧复合梭曼中毒PC12细胞中白介素-4、白介素-8(IL-4、IL-8)和酪氨酸蛋白激酶(TPK)活性的变化。方法采用ELISA法和放射免疫技术检测缺氧复合梭曼中毒PC12细胞的IL-4、IL-8和TPK活性。结果单纯梭曼中毒PC12细胞胞浆、胞核的TPK以及IL-4、IL-8活性在中毒2 h升高,12 h活性水平最高,24 h活性降低。缺氧复合梭曼中毒组PC12细胞胞浆、胞核的TPK以及IL-4、IL-8活性在缺氧中毒2 h升高,6 h最高,12 h活性降低,各时相点均明显高于正常对照组。结论提示酪氨酸蛋白激酶、IL-4、IL-8参与缺氧复合梭曼中毒所致脑损伤的发病机制。

Objective To explore the changes of interleukin-4, 8 and TPK in PC12 cells exposed to hypoxia and soman intoxication. Methods PC 12 cells were randomized into 4 groups： control, hypoxia only, soman only, hypoxia plus soman. The activity of IL-4, IL-8 was measured by ELISA and the activity of tyrosine protein kinase in cells were detected by RIA. Results The activity of IL-4, IL-8 in PC12 cell supernatant and TPK in PC12 cell cytoplasm, nucleus were increased in 2 h and reached the highest at 12 h, decreased at 24 h in soman group, correspondingly the activity of IL-4, IL-8 in PC12 cell supernatant and TPK in PC12 cell cytoplasm, nucleus were increased at 2 h and reached the highest at 6 h, decreased at 12 h in soman plus hypoxia group, all higher than that of control. Conclusion TPK, cytokine and its correlated signal pathway lead to an important modulatory role in the brain damage after combined soman and hvnoxia injury.