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人白介素24重组蛋白的表达及其抗肿瘤机理的研究 被引量：12

The Expression of Recombinant Protein of Human Interleukin-24 and its Anti-tumor Mechanism
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摘要 将人白细胞介素24(hIL24)的cDNA序列克隆至原核高效表达载体pET21a(+)上,经IPTG诱导后,在大肠杆菌中获高效表达,经纯化、复性后的rhIL24蛋白具有抑制HeLa细胞生长,诱导HeLa细胞凋亡,刺激外周血单核细胞分泌IL6、TNFα、IFNr,抑制血管形成的功能。初探了rhIL24蛋白能通过下调抗凋亡因子bcl2表达,激活线粒体凋亡途径引起肿瘤细胞凋亡的机理。 The hIL24 cDNA sequence was cloned into prokaryotic high expressive vector pET-21a（ ＋ ） and recombinant hIL24 was expressed in E. coli with IPTG induction. The purified recombinant hIL24 exhibits following functions in HeLa cell： inhibiting cell growth, inducing apoptosis, inducing PMBC to secrete IL-6,TNF-a,IFN-r and inhibiting blood vessel formation. Our preliminary results suggest that the apoptosis induced by rhIL24 is through down-regulating expression of anti-apoptosis factor Bcl-2 and activation of mitochondria apoptosis pathway.
作者 叶震敏 朱晔涵 盛伟华 缪竞诚 危少华 吴康 杨吉成
机构地区 苏州大学医学院细胞与分子生物学教研室 苏州大学附属第一人民医院
出处 《生物工程学报》 CAS CSCD 北大核心 2005年第5期719-724,共6页 Chinese Journal of Biotechnology
基金 苏州大学医学发展基金资助项目(No.EE134517)。~~
关键词 人白介素24重组蛋白 HELA细胞 抗肿瘤机理 recombinant human IL-24 protein, HeLa cell, anti-tumor mechanism
分类号 R73-3 [医药卫生—肿瘤]
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参考文献12

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生物工程学报
2005年 第5期

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