CO_2对牟氏角毛藻高密度培养的影响

Effects of CO_2 on High Density Culture of Chaetoceros mulleri

以牟氏角毛藻为材料,通过对比实验研究了CO2和CO2通入方式对光合生物反应器中高密度培养的牟氏角毛藻的生长速度和细胞密度的影响;并以水体pH值为指标,控制CO2的通入数量,进而研究了牟氏角毛藻在我们自行研制的卧式光合生物反应器中的生长情况。结果表明细胞悬浮培养达到一定密度之后,CO2的供应,可以有效地提高生长速度和细胞密度。对CO2通入方式的实验结果表明,不同的CO2通入方式,产生的效果不同,其中以空气和纯CO2混合后通入,效果最好。

The effect of CO2 and the manner of CO2 offer on the growth rate and maximual cell density of uhro-high density culture of Chaetoceros mulleri in the photobioreactor were studied in the work. The amount of CO2 offered to the culture was controlled by the parameter of pH value in the culture. Furthermore the growth kinetics of Chaetoceros mulleri in the photobioreactor was studied. The results showed requirement of CO2 by the cells and the increase of pH in the culture were the key limiting factors to the growth, when a high cell concentration in the culture was reached. The offer of CO2 could improve the statute of CO2, could control the pH in the culture and increase the growth rate and maximum cell density. The results from the experiments of CO2 offer manner showed different efficiency to growth was resulted from differences of CO2 offer manner. The best way is mixing the CO2 and air before the CO2 was offered to the culture.