摘要
目的:对SPF(SpecificPathogenFree)仔猪断奶前后的肠道菌群组成进行动态监测,并以普通饲养仔猪(自然分娩)为对照作相关分子微生物生态学分析。方法:利用剖腹取胎的方式将仔猪从母体子宫中取出放于屏障系统中饲养,于7日龄时转移至消毒的开放式环境中,14日龄断奶,分别于10日龄、16日龄和22日龄以及32日龄采集仔猪新鲜粪便样品。提取粪便样品中细菌总DNA,获得其ERIC-PCR指纹图谱,再将其中一个样品的ERIC-PCR产物以地高辛标记为混合探针通过杂交对指纹图谱上DNA条带序列的异同进一步比较。结果:SPF仔猪和普通仔猪在断奶后肠道菌群的组成发生较大变化,而且SPF仔猪的肠道菌群和普通仔猪有显著差异。
Objective： To monitor and characterize the structural features of intestinal microbial communities in fecal samples of SPF（ specific pathogen free） piglets and conventional piglets. Methods： The newborn piglets were transferred from the womb into a barrier system immediately after caesarean operation , moved into an open sterile environment on the 7^th day,and was weaned on the 14^th day. Fecal samples were collected on the 10^th , 16^th , 22^th and 32^th days. The genomic DNA of bacteria was extracted from each sample and ERIC - PCR amplification was then carried out. The PCR fingerprints were southern - blotted with a mixed dig - tagged probe from the labeled ERIC - PCR product of one sample. Results： The banding - patterns of samples of SPF piglets and conventional piglets elucidate poor similarities before, during and after weaning respectively. Simultaneously there really existed much difference in the aspect of the structural features of intestinal microflora between SPF piglets and conventional piglets on the DNA level.
出处
《上海交通大学学报（农业科学版）》
2005年第3期256-260,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
国家自然科学基金项目(30370031)
