大脑中动脉闭阻脑缺血模型大鼠的建立与评价

Establishment and evaluation of middle cerebral artery occlusion (MCAO) models in SD rats

目的建立具有良好的重复性和稳定性的局灶性脑缺血模型。方法采用低位开颅窗手术,用损伤面积较小的热凝法闭阻大脑中动脉(MCA)建立脑缺血模型。并在造模后将动物按缺血时间随机分为3d、7d、14d和21d组,采用神经功能缺损评分(NSS)、TTC染色与HE染色动态观察神经功能缺损征、脑梗死体积及观察病理改变情况。结果造模后动物均出现相当程度的功能缺损[(2.78±0.44)分^(1.67±0.50)分]、脑梗死[(197.05±23.38)分^(135.15±13.18)分]和病理改变。神经功能缺损征与脑梗死体积在缺血后3d组与7d组比较有明显的改善和缩小(P<0.01),7d之后变化趋于平稳(P>0.05)。结论本方法操作简便,所建立的脑梗死模型具有良好的稳定性和重复性。

Objective To establish the model of focal cerebral ischemia with favorable reproducibility and stability, and investigate the syndrome of neurological severity, the volume of cerebral infarction and the pathological changes after different ischemic time. Methods To establish cerebral ischemia model, the low-setting skull window was performed, and the middle cerebral artery （MCA） was obstructed by thermocoagulation with less area of injury. These models were divided randomly into four group according to the different ischemic time（ 3d ,7d, 14d and 21 d）. Neurological severity score （NSS）, triphenyltetrazolium chloride （TTC） staining and HE staining were adopted to observe dynamically the syndrome of neurological severity, the volume of cerebral infarction and the pathological changes. Results The neurological severity （ 2.78 ±0.44 - 1.67 ± 0.50 ）, cerebral infarction （ 197.05 ±23.38 - 135.15 ±13.18 ） and pathological changes appeared distinctly in all rats after operation. The syndrome of neurological severity ameliorated and the volume of cerebral infarction deflated significantly comparing 3d group with 7d group after ischemia （ P〈0.01 ）, and the diversity went stable after 7 days （ P〉 0.05）. Conclusion The method is simple and convenient, and the MCAO model has favorable reproducibility and stability.