肉桂离体胚超低温保存研究

Study of Cryopreservation on Cinnamomum cassia Excised Embryos

对不同含水量肉桂离体胚进行超低温(LN2)保存,采用不同冷冻方式、解冻方式及防冻剂预处理,并测定超低温保存前后的脱氢酶、电导率、α-淀粉酶生理生化指标.结果表明:超低温保存的技术关键是减少保存材料在脱水过程中所受的伤害,以及采用适宜的冷冻和解冻方式和添加适宜的防冻剂.防冻剂预处理可以适当提高保存含水量,增加含水量范围.肉桂离体胚没有添加防冻剂时最适宜保存含水量为30%～40%(w:w),采用快冻快解处理.防冻剂预处理后的保存效果比没有防冻剂预处理的更佳,采用缓冻快解或快冻缓解处理较为适宜.

By taking different freezing method, thawing method and cryoprotectants as treatment factors, the cryopreservation of Cinnamomum cassia excised embryos with different moisture content was studied, and the dehydrogenase activity, the conductivity and a-amylase activity were measured and contrasted after cryopreserving with before. The results showed that the key of cryopreservation technology must be succeeded in reducing the harm of conservation materials during the desiccation and cryopreservation process, by taking the optimum magnitude and type of cryoprotectants, freezing and thawing method. The preservation effects adding the suitable antifreezes broadened the optimum cryopreserving moisture content level, and then enlarged the cryopreserved scale of moisture content. Cryopreserved without cryoprotectants, the suitable moisture content of the C. cassia excised embryos is 30% ～ 40%. And taking quick freezing-quick thawing methods was better than others. Otherwise, the effect cryopreserved with cryoprotectants was much better than that without cryoprotectants, associated with mild freezing- quick thawing methods or quick freezing-mild thawing methods.