具有复合靶向抗癌功能的纳米高分子材料

PREPARATION OF B1-TARGET ANTICANCER DRUGSIMMUNOLOGICAL MAGNETIC ADRIAMYCIN NANOPARTICLES AND ITS EVALUATION IN VITRO

用氧化还原法制备出具有复合靶向抗癌功能的纳米高分子材料阿霉素免疫磁性毫微粒，并对制备条件进行优化，用扫描电镜和光子相关光谱对产物进行了定性和定量表征，并进行了体外抑瘤试验和体外磁导向定位试验。

Adriamycin and anti-human mammary cancer monoclonal antibody (McAb) were conjugated to the magnetic iron-dextran nanoparticles by the periodate oxidation-borohydride reduction procedure. The conjugates were separated out by gel filtration chromatography on Sephacryl S-300 and characterized by SEM and laser scattering system. The mean diameter of nanoparticles was 216 nm and the molar ratio of Ab∶Dex∶Adr in conjugate was 1∶5∶94. The conjugates prepared by this procedure had high immunological activity evaluated by ELISA and did not flocculate in physiological buffer. The nanoparticles could well bind the cancer cells and killed 80% of them in vitro, showing that the conjugate had significant retention of biological activity of the antibody. A magnetic field was kept on the simulated blood capillaries. The nanoparticles flowed in the capillaries could be 100% retained in the magnetic area and removed if the magnetic field disappeared, showing that they had high magnetic responsibility and superparamagnetism.