纳米高分子材料在抗癌药物控释方面的应用

APPLICATION OF NANOPARTICLES IN CONTROLLED RELEASE OF ANTICANCER DRUGS: PREPARATION AND EVALUATION IN VITRO OF METHOTREXATE O CARBOXYMETHYLATE CHITOSAN NANOPARTICLES

本研究用“等电临界法”制备Ｏ－羧甲基甲壳胺甲氨喋呤毫微粒并在模拟人工胃液、肠液和１％新鲜小鼠血清中进行控释试验，结果表明抗癌药物与载体材料不同的投料比，交联剂用量及释药介质均对毫微粒的释药速度产生影响。

Nanoparticles (Np) of Methotrexate (MTX) were prepared using O-Carboxymethylate chitosan (O-CMC) as wall-forming materials, and an isoelectric-critical technique under ambient conditions. Drug controlled releases were studied in several media including simulated gastric fluid, intestinal fluid and 1% fresh mice serum. It was found that acidic media provided a fast release rate than neutral media. The effect of MTX/O-CMC ratio and amount of cross-linking agent on drug release in different media were evaluated. The changes of size and effective diameter of O-CMC Np were detected by SEM and laser light scattering system before and after drug release. The O-CMC nanoparticles might constitute an attractive alternative to other anticancer drugs and enzyme carriers.