摘要
本试验用卵巢癌细胞株,采用三磷酸腺苷法(ATP法)分别观察四种化疗增敏剂即三苯氧胺、克罗米酚、环孢菌素A及维拉帕米对顺铂(CDDP)或紫杉醇(Taxol)的体外增效作用。结果显示,各增敏剂在较低浓度(1μg/ml)情况下,单用时均无明显抑瘤作用,但与CDDP或Taxol合用时能增强其在一定剂量下对肿瘤细胞的杀伤作用。各增敏剂在较高浓度(5μg/ml)下,三苯氧胺、克罗米酚及环孢菌素A单用有一定抑瘤作用,此时四药与CDDP或Taxol合用时抑制率增高且高于化疗药物或化疗增敏剂单用时的抑制率。增效作用以三苯氧胺和环孢菌素A最强,故提出可将三苯氧胺或环孢菌素A作为CDDP和Taxol的增效剂用于卵巢癌化疗。另外本文检测该细胞株雌激素受体(FR)为阴性,提示三苯氧胺亦可试用于ER(一)的患者。
The enhanced effect of 4 agents(tamoxifen clomiphene,cyclosporin A and verapamil)wasobserved in vitro on the cytotoxicity of DDP and Taxol on the ovarian cancer cell line by ATP assay. Theresults showed that 4 agents had no cytotoxicity themselves when added alone to the cell line at lower con-centration(1 μg/ml),but they showed enhanced effcets when added with DDP or Taxol.However,athigher concentration(5 μg/ml),tamoxifen,clomiphene and cyclosporin A showed inhibitory effects whenused alone,all 4 agents showed an increased inhibitory rate when they were added with DDP or Taxol atthis concentration and the inhibitory rates were higher than that of chemotherapeutic drtigs or these 4 agentsalone. Either tamoxifen or cyclosporin A showed the stronger effect than that of the other two agents,theymay be used in the chemotherapy of the patients with ovarian cancer as an enhtqncer of DDP and Taxol.Wemeasured estrogen receptor(ER)of this cell line by immunohistochemical method and the result was nega-tive,it was suggested that tamoxifen may be also used in ER-negative ovarian cancer clinically.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1996年第3期161-163,共3页
Chinese Journal of Cancer
基金
国家自然科学基金