四种化疗增敏剂的体外增效作用

A STUDY ON THE ENHANCED EFFECT OF 4 AGENTS ON THE CYTOTOXICITY OF DDP AND TAXOL IN VITRO

本试验用卵巢癌细胞株，采用三磷酸腺苷法（ＡＴＰ法）分别观察四种化疗增敏剂即三苯氧胺、克罗米酚、环孢菌素Ａ及维拉帕米对顺铂（ＣＤＤＰ）或紫杉醇（Ｔａｘｏｌ）的体外增效作用。结果显示，各增敏剂在较低浓度（１μｇ／ｍｌ）情况下，单用时均无明显抑瘤作用，但与ＣＤＤＰ或Ｔａｘｏｌ合用时能增强其在一定剂量下对肿瘤细胞的杀伤作用。各增敏剂在较高浓度（５μｇ／ｍｌ）下，三苯氧胺、克罗米酚及环孢菌素Ａ单用有一定抑瘤作用，此时四药与ＣＤＤＰ或Ｔａｘｏｌ合用时抑制率增高且高于化疗药物或化疗增敏剂单用时的抑制率。增效作用以三苯氧胺和环孢菌素Ａ最强，故提出可将三苯氧胺或环孢菌素Ａ作为ＣＤＤＰ和Ｔａｘｏｌ的增效剂用于卵巢癌化疗。另外本文检测该细胞株雌激素受体（ＦＲ）为阴性，提示三苯氧胺亦可试用于ＥＲ（一）的患者。

The enhanced effect of 4 agents(tamoxifen clomiphene,cyclosporin A and verapamil)wasobserved in vitro on the cytotoxicity of DDP and Taxol on the ovarian cancer cell line by ATP assay. Theresults showed that 4 agents had no cytotoxicity themselves when added alone to the cell line at lower con-centration(1 μg/ml),but they showed enhanced effcets when added with DDP or Taxol.However,athigher concentration(5 μg/ml),tamoxifen,clomiphene and cyclosporin A showed inhibitory effects whenused alone,all 4 agents showed an increased inhibitory rate when they were added with DDP or Taxol atthis concentration and the inhibitory rates were higher than that of chemotherapeutic drtigs or these 4 agentsalone. Either tamoxifen or cyclosporin A showed the stronger effect than that of the other two agents,theymay be used in the chemotherapy of the patients with ovarian cancer as an enhtqncer of DDP and Taxol.Wemeasured estrogen receptor(ER)of this cell line by immunohistochemical method and the result was nega-tive,it was suggested that tamoxifen may be also used in ER-negative ovarian cancer clinically.