超氧化物歧化酶防治口腔粘膜炎性损害的动物实验研究——SOD清除氧自由基的直接证据

Protective Effect of Superoxide Dismutase(SOD) Against Paraquat (PQ) Induced Inflammation in Animal Oral Mucosa: A Direct Evidence of SOD Scavenging Oxygen Free Radical(OFR)

为了解超氧化物岐化酶（ＳＯＤ）防治甲基紫精（ＰＱ）诱导的口腔粘膜炎性损害的机理，采用目前唯一能直接检测自由基的电子自旋共振（ＥＳＲ）技术，对金黄地鼠颊囊炎性组织中的自由基进行定性、定量研究，同时分析了ＳＯＤ的作用效能。结果表明：炎性组织中有３种饱和功率不尽相同的自由基讯号，其中ｂ峰属于氧自由基。ＳＯＤ能使氧自由基相对含量显著降低，ＳＯＤ组清除氧自由基的能力是对照组的６５倍以上。

In order to understand the mechanisms of SOD for its anti inflammation function,a direct technique,electron spin resonance(ESR) spectroscopy,was used to investigate the free radical(FR) produced in hamster oral mucosa.The efficacy of SOD of scavenging OFR was also analyzed.The results showed that the spectra of the inflammatory tissues exhibited three different signals with different power saturation. Among these signals,signal b was most likely that of OFR. The relative OFR intensity was significantly reduced by SOD(P<0.05).The ability of scavenging OFR in SOD group was over 65 fold than that of control group.