不同钙负荷剂量对大鼠生长及钙铁锌水平影响

Effect of different calcium load on growth and lever of Ca,Fe,Zn in rats

目的以不同负荷剂量的钙饲料喂养正常生长发育期的大鼠,研究过量钙补充对机体生长发育、钙吸收代谢及对机体铁锌元素水平的影响。方法将初断乳的Wistar雄性大鼠按体重随机分成4组,其中本底组于喂养开始时全部处死,作为钙代谢实验的本底值。其余3组分别饲以不同钙负荷剂量的饲料,进行为期28 d的生长实验,随后分别进行3 d的钙代谢实验。结果饲料中不同钙负荷剂量对大鼠的生长发育有影响,其中体重增长缓慢,股骨重量增加,但股骨长度并未增加,提示在生长发育期长骨过早发生钙化不利于身高的增长。过量钙喂养使大鼠脏器、器官的脏体系数发生改变,对睾丸、肾脏等器官有一定危害;机体对钙的吸收、利用率下降,造成补钙剂的浪费,增加机体代谢负担,同时使血清铁、锌浓度降低,血清钙浓度增高,尿钙增加,是肾结石的潜在危险因素。结论给正常生长发育期的大鼠补充过量钙剂,不仅对其生长发育有影响,同时对某些脏器有危害。过量钙剂干扰了机体对钙、铁、锌等营养元素的吸收利用。

Objective To Study the influence of excessive calcium supplyment on the growth of rats, metabolize of calcium and lever of iron and zinc. Methods 40 weaned Wistar rats were divided randomly and equally into 4 groups according to the weight. One of them was background group which was killed before fed. The others were fed by different calcium content for 28 days then were engaged in metabolize of calcium experiment. Results Rats＇ growth were influenced by the different calcium content, which inclued rats＇ weight gained slowly. The weight of the thighbone was increased but the length of it wasn＇ t increased. The bone were calcified too early by excessive calcium intake. Excessive calcium intake made the viscera coefficient of rats changed, and had certain harm to testicle and kidney and so on. The absorbability and using of calcium were decreased, which wasted calcium and increased metabolizable burden of body. At the same time, it made the concentration of serum zinc and serum iron depressed, but the serum calcium went up. High uric calcium was the latent dangerous factor of nephrolith. Conclusion The excessive calcium influenced the growth of rats and harmed certain viscera. And it disturbed the absorbability and using of calcium, iron and zinc.