人蛔虫体腔液对人肠上皮细胞的毒性作用

Toxic effect of Ascaris lumbricoides body fluid on intestinal epithelial cells

目的探讨人蛔虫体腔液(Ascaris bodyfluid,ABF)对人肠上皮细胞株(HCT-8)的毒性作用及作用浓度和时间的关系.方法以体外细胞培养方法观察ABF对HCT-8细胞的毒性作用,比较不同浓度ABF作用下不同时间HCT-8细胞的死亡率和形态结构.结果HE染色显示,当ABF浓度为800μg/ml时,HCT-8细胞的毒性作用最大,除浓度为12.5μg/ml的实验组,各实验组(25～8μg/ml)的毒性均高于对照组.结论ABF对HCT-8细胞所产生毒性作用在一定程度上表现出浓度和作用时间的依赖关系,并诱导细胞凋亡的发生.

Objective To study the toxic effect of body fluid of Ascaris lumbricoides（ABF）on human intestinal epithelial cells（HCT-8 cells）and how the ABF concentration and inducing time could be related to the toxicity. Methods Survival rates, morphology and function of HCT-8 cells were compared in different ABF concentrations（i.e., 30,100,400μg/ml）and different time in vitro. Results The observation from HE stain showed the most toxic effect of HCT-8 cells at 800μg/ml for 8 h. The toxicity of HCT-8 cels in each experiment group（25-800μg/ml）was higher than that of matched control except 12.5 %μg/ml. Conclusion The toxicity induced in vitro by ABF implies a dose-and time-dependent pattern in some extension. Meanwhile, ABF induced the apoptosis of HCT-8 cells.