重金属离子对凡纳滨对虾肝胰脏、鳃丝和血液SOD活力的影响

Effects of heavy metal ions on SOD activity of Litopenaeus vannamei hepatopancreas,gill and blood

研究了3种重金属离子(Cu2+、Zn2+、Cd2+)在96h内对凡纳滨对虾(Litopenaeusvannamei)对肝胰脏、鳃丝和血液超氧化物歧化酶(SOD)活力的影响.结果表明,凡纳滨对虾SOD活力在3种重金属离子作用下随取样时间变化显著(P<0.05),Cu2+在实验浓度范围内(0.1～1mg·L-1),肝胰脏、鳃丝和血液的SOD活力随时间延长呈一峰值变化,Zn2+在10mg·L-1时对肝胰脏表现为显著抑制作用,Cd2+在0.5mg·L-1时对肝胰脏和鳃丝起显著抑制作用,0.25mg·L-1对鳃丝SOD活力无显著变化(P>0.05),其他浓度Zn2+(<10mg·L-1)、Cd2+(<0.25mg·L-1)对各组织器官SOD活力的影响随时间延长均呈现先升高后下降的趋势.3种重金属离子对凡纳滨对虾肝胰脏、鳃丝、血液SOD活力的影响呈现明显的剂量时间效应关系.其SOD活力大小顺序为肝胰脏>鳃丝>血液,3种重金属离子对凡纳滨对虾伤害大小顺序为Cd2+>Cu2+>Zn2+.

This paper studied the effects of Cu^2＋ , Zn^2＋ and Cd^2＋ on the superoxide dismutase （SOD） activity of Litopenaeus vannamei hepatopancreas, gill and blood. The results showed that the SOD activity changed significantly with prolonged exposure of these ions （P 〈 0.05 ）. The SOD activity of all test objectives changed with a single peak under the exposure of 0. 1 - 1 mg Cu^2＋ · L^-1, that of hepatopancreas and of hepatopancreas and gills was inhibited obviously under 10 mg Zn^2＋· L ^- 1 and 0.5 mg Cd^2＋· L^- 1, respectively, while 0.25 mg Cd^2＋· L^- 1 had no significant effect on that of gill. The SOD activity of hepatopancreas, gill and blood all increased first and then decreased under the prolonged exposure of 〈 10 mg Zn^2＋ · L^-1 and 〈 0.25 mg Cd^2＋· L^-1. There was an obvious dose-time response relationship between test metal ions and SOD activity. The SOD activity was decreased in order of hepatopancreas〉gill〉blood, while the toxicity of test metal ions was in order of Cd^2＋ 〉Cu^2＋ 〉Zn^2＋ .