近干骺端骨延长侧骺板细胞核DNA的实验研究

Experimental research on DNA content of the cell nuclei in the lengthening side physis after near metaphyseal osteotomy

为探讨用有一定张力的外固定架行干骺端延长,是否会影响同侧骺板的生长发育,作者用自制Ilizarov外固定架对35只山羊施行左胫骨骨干延长术。术后第7、14、21、28、35、49和63天分批各处死5只。延长侧骺板脱钙行Feulgen染色、VIDAS测定DNA。发现延长侧骺板细胞DNA受压应力影响较小,核DNA为3C和4C;术后35天去架后摄片见骺线存在。结果表明,近干骺端骨延长术虽然安全,但仍须注意减少压应力对骺板的损伤。

In order to study the influece of the diaphyseal distraction upon further growth of the lengthening side physis, We applied the modified Ilizarov- type external fixator to the left tibiae of thirty-five goats. Diaphyseal corticotomies were performed, and distraction was carried out by using the Ilizarov's method. A group of 5 goats each was sacrificed 7,14,21, 28,35,49 and 63 days postoperativ-ely. Specimens of the lengthening side epiphysis for DNA quantitation VIDAS were decalcified with 5% EDTA and Feulgen stained. There was little influence subject to the Tension-Stress in lengthening side epiphysis as the mean DNA content corroborated its cell nuclei in 3C - 4C region. The hypertrophic lay-er of physis on the lengthened side was thicker than that on the control side while the lines of epiphysis in roentgenography was still observable after removal of external fixator at 35 days post lengthening. Although lengthening by metaphyeal osteotomy was safe,the damage to the physis by Tension-Stress should be minimized.