黄芪注射液对急性放射性脑损伤大鼠认知能力及行为变化的干预效应

Interventional effect of astragale parenteral solution on the cognitive ability and behavioral changes of rats with acute radioactive injury

目的:观察黄芪注射液对大鼠急性放射性脑损伤后脑内一氧化氮含量及其认知能力和行为变化的影响。方法:实验于2004-08/2005-06在福建医科大学附属协和医院鼠类实验室完成。选择4个月龄健康Wistar雄性大鼠120只,随机分为模型组、正常对照组及治疗组3组,每组40只。模型组及治疗组动物均置于放射系数为零的暗箱内,入60Co治疗仪(放射源)进行照射,平均放射剂量为0.8Gy/min,每野的放射剂量为2.25Gy,总剂量为4.5Gy/min,照射160～170s。对照组设假辐射对照。照射1h后模型组和治疗组均腹腔注射生理盐水100mg/(kg·d),综合治疗组腹腔注射黄芪注射液100mg/(kg·d),连续给药2周。采用单程逃避实验观察60C0照射后不同时期大鼠认知能力变化,并以Griess比色法检测60C0照射后不同时期大鼠脑内一氧化氮含量变化。结果:120只大鼠均进入结果分析,无脱落。①以单程逃避实验观察大鼠认知能力:照射前各组完成逃避的百分比差异性无显著性。经60C0照射后,各相同的时间段内,模型组、治疗组比对照组完成逃避的百分率明显减少(F=169.716-286.04,P<0.05)。②3组一氧化氮含量比较:模型组、治疗组额叶和海马区域比对照组明显升高(F=52.4-124.9,77.9-200.8,P<0.05)结论:腹腔注射黄芪注射液能改善放射性脑病大鼠的认知能力,减少脑内一氧化氮的含量,达到保护放射性脑损伤的作用。

AIM： To observe the effect of astragale parenteral solution on the content of nitric oxide in brain and the cognitive ability and behavioral changes in rats after acute brain injury. METHODS： The experiment was carried out in the rat laboratory of Union Hospital affiliated to Fujian Medical University from August 2004 to June 2005. A total of 120 healthy male 4-month-old Wistar rats were randomized into treatment model group （n=40） , normal control group （n=40） and treatment group （n=40） . Rats in the model group and treatment group were placed in the dark chamber with the radiation coefficient of 0, and irradiated with the 60C0 treatment meter （radiation source） , the average radiation dosage was 0.8 Gy per minute, 2.25 Gy for each field, the total dosage was 4.5 Gy per minute for 160-170 s. The control group was shamradiation control. 1 hour after irradiation, the rats in the model group and control group were treated with intraperitoneal injection of saline （100 mg/kg per day） , and those in the treatment group were given intraperitoneal injection of astragale parenteral solution （100 mg/kg per day） , they were treated for 2 continuous weeks. The changes of cognitive ability at different period of irradiation were observed with the one-way avoidance test, and the changes of nitric oxide were detected with Griess chromatometry. RESULTS： All the 120 rats were involved in the analysis of results without deletion. ① Cognitive ability observed with the one-way avoidance test： Before irradiation, the percentage of complete avoidance was insignificantly different among the groups; After 60C0 irradiation, the percentage of complete avoidance at the same time point was obviously decreased in the model group and treatment group as compared with the control group （F= 169.716-286.04, P〈0.05） . ② Comparison of the nitric oxide content among the 3 groups： The nitric oxide contents in frontal lobe and hippoeampus were obviously increased in the model group and treatment group than in the control group （F=52.4-124.9, 77.9-200.8, P〈0.05） . CONCLUSION： The intraperitoneal injection of astragale parenteral solution can ameliorate the eognitive ability and deereae the nitric oxide content in brain of rats with acute encephalopathy, radioactive brain injury.