核仁组成区相关蛋白对前列腺腺癌病理诊断的价值

Significance of argyrophil staining for nucleolar organizer regions (AgNORs) to pathologic diognosis of prostatic adenocarcinoma

作者应用胶体银染色(AgNORs)技术对7例前列腺腺癌及其癌周组织和20例前列腺增生症的细胞核仁变化进行了观察分析。结果显示前列腺腺癌细胞的核仁组成区相关蛋白AgNORs平均为4.322±0.269个,核仁平均面积为6.236±2.199μm^2,核仁最大直径2.1μm,且核仁有不同程度的畸形。癌周组织和前列腺增生症AgNORs分别为1.820±0.683个和2.284±0.383个,核仁平均面积分别为1.829±0.146μm^2和1.673±0.216μm^2,核仁最大直径均为1.1μm,核仁均无畸形。腺癌与癌周组织及前列腺增生症之间差异有极显著意义(P<0.01)。癌周组织与前列腺增生症间差异无显著意义(P>0.05)。作者认为核仁体积和AgNORs计数的变化,对前列腺腺癌的病理诊断和鉴别诊断有重要价值。

Argyrophil staining method for nucleolar organizer regions (AgNORs)was used in 7 pa-tients with well-differentiated prostatic adenocarcinoma (PAC) and tissue-surrounded adenocarcinoma (TSC) and 20 patients with prostatic hyperplasia (PH). The mean count of AgNORs of PAC was 4. 322±0. 269, and the mean area of nucleolus 6. 236±2. 199μm^2its largest diameter was 2. 1μm and its size and shape showed some deformity. The mean count of AgNORs of tissue-surrounded PAC and PH was 1. 820±0. 683 and 2. 284±0. 383 respectively. The mean area of their nucleolus was 1. 892±0. 146μm^2 and 1. 673±0. 216μm^2. The largest diameter was the same 1. 1μm. Statistically, there was highly significant between PH and TSC (P>0.05). The study revealed that the changes of size of nucle-olus and AgNORs count are significant in pathologic diagnosis and differential diagnosis of PAC.