祁州漏芦乙醇提取物对D-半乳糖所致衰老小鼠学习记忆的影响(英文)

Effects of alcohol extract of Qizhou radix echinopsis on learning and memory in senile mice induced by D-galactose

背景:脂质过氧化损害可导致学习记忆障碍,而漏芦能抑制脂质过氧化,具有提高细胞免疫功能、抗自由基过氧化等作用。目的:通过脑组织中过氧化脂质及脂褐质含量的变化,探讨漏芦乙醇提取物对D-半乳糖所致衰老小鼠学习记忆的影响。设计:随机对照实验。单位:沈阳药科大学药学院药理教研室,中国医科大学第一临床学院中医教研室,沈阳药科大学中药学院天然药化教研室。材料:实验于1994-02/10在沈阳药科大学药学院药理教研室完成。选取清洁级昆明种小鼠185只,随机分成7组:漏芦乙醇提取物3.125g/(kg·d)组30只、漏芦乙醇提取物1.56g/(kg·d)组30只、漏芦乙醇提取物0.78g/(kg·d)组30只、漏芦乙醇提取物0.39g/(kg·d)组15只、吡拉西坦组15只、空白对照组30只、模型组35只。方法:漏芦乙醇提取物3.125,1.56,0.78,0.39g/(kg·d)组分别灌服漏芦乙醇提取物3.125,1.56,0.78,0.39g/(kg·d),吡拉西坦组灌服吡拉西坦0.2g/(kg·d),空白对照组和模型组分别灌服同容积蒸馏水。除空白对照组外,其余各组小鼠每天均皮下注射50g/LD-半乳糖0.5mL/只,连续40d,建立小鼠衰老模型。40d后,采用跳台法及避暗法测定各组小鼠的学习记忆能力。主要观察指标:①各组小鼠在跳台法检测中的错误次数。②各组小鼠在避暗法检测中的错误次数及潜伏期的变化。③各组小鼠脑组织过氧化脂质及脂褐质含量生成的比较。结果:实验纳入小鼠185只,共169只进入结果分析。①各组小鼠在跳台法检测中的错误次数:与模型组比较,漏芦乙醇提取物3.125,1.56,0.78g/(kg·d)组均明显减少[(1.68±1.25),(0.92±0.73),(0.78±0.79),(0.97±0.67)次,P<0.01,P<0.001,P<0.01]。②各组小鼠在避暗法检测中的错误次数及潜伏期的变化:与模型组比较,漏芦乙醇提取物3.125,1.56,0.78g/(kg·d)组均明显减少(P<0.05或0.01);且潜伏期均有所延长(P<0.05或0.01)。③各组小鼠脑组织过氧化脂质及脂褐质含量生成的比较:与模型组比较,漏芦乙醇提取物3.125,1.56,0.78g/(kg·d)组均明显减少(P<0.05或0.01)。结论:漏芦乙醇提取物具有抗衰益智作用,其抗自由基过氧化、减少脑组织中过氧化脂质及脂褐质生成可能与其改善记忆障碍作用有关。

BACKGROUND： Lipid peroxidation results in disturbance of learning and memory, but radix echinopsis inhibits lipid peroxidation and acts on improving immune function of cell and resisting peroxidation of free radical. OBJECTIVE： To probe into the effects of alcohol extract of radix echinopsis on learning and memory of senile mice induced by D-galactose through the changes in lipid peroxide （LPO） and lipofuscin contents. DESIGN： Randomized controlled experiment was designed. SETTING： Teaching-Research Room of Pharmacology in School of Pharmacy of Shenyang Pharmaceutical University, Teaching-Research Room of Chinese Medicine of the First Clinical College of China Medical Universi- ty, Teaching-Research Room of Natural Medicine and Chemistry in School of Traditional Chinese Medicine of Shenyang Pharmaceutical University. MATERIALS： The experiment was performed in Teaching-Research Room of Pharmacology in School of Pharmacy of Shenyang Pharmaceutical University from February 1994 to October 1994. Totally 185 Kunming mice of clean grade were employed and randomized into 7 groups, named alcohol extract of radix echinopsis 3.125 g/（kgod） group [3.125 g/（kgod） group]]（30 mice）, alcohol extract of radix echinopsis 1.56 g/（kgod） group [1.56 g/（kgod） group] （30 mice）, alcohol extract of radix echinopsis 0.78 g/（kgod） gavup [0.78 g/（kgod） group] （30 mice）, alcohol extract of radix echinopsis 0.39 g/（kgod） group [0.39 g/（kgod） group] （15 mice）, piracetam group （15 mice）, blank control （30 mice） and model group （35 mice）. METHODS： Alcohol extract of radix echinopsis was infused in each of the groups of alcohol extract of radix echinopsis at 3.125, 1.56, 0.78 and 0.39 g/kgod successively. In piracetam group, piracetam 0.2 g/kgod was applied. Distilled water of equal volume was infused in blank control and model group respectively. Except hlack control, in rest groups, 50 g/L Dgalactose was injected subcutaneously everyday, 0.5 mlJmouse, continuously for 40 days, by which, senile mouse model was prepared. Forty days later, learning and memory were determined in each group with step down test and step through test. MAIN OUTCOME MEASURES： ① Errors in step down test in each group ② Changes in errors and latent phase in step through test in each group ③ Comparison of LPO and lipfuscin contents in brain tissue of each group RESULTS： Totally 185 mice were employed in the experiment, totally, 169 mice entered result analysis. ①Errors in step down test in each group： compared with model group, the error in 3.125,1.56,0.78 g/ （kgod）groups was reduced remarkably [（1.68±1.25）, （0.92±0.73）, （0,78± 0.79）, （0.97±0.67）times,P 〈 0.01 ,P 〈 0.001 ,P 〈 0.01] successively. ② Changes in errors and latent phase in step through test in each group： compared with model group, the error in 3.125,1.56,0.78 g/（ kgod ） groups was reduced remarkably（P 〈 0.05 or P 〈 0.01） successively and the latent phase was prolonged successively（P 〈 0.05 or P 〈 0.01）. ③ Comparison of LPO and lipfuscin contents in brain tissue of each group： compared with model group, those in 3.125,1.56,0.78 g/（kgod）groups were reduced remarkably successively（P 〈 0.05 or P 〈 0.01）. CONCLUSION： Alcohol extract of radix echinopsis acts on resisting aging, benefiting intelligence, being against peroxidation of free radical and reduces the production of LPO and lipofuscin in brain tissue, which is probably attributed to its action on improving memory disturbance.