黄芪及丹参对防治甘油致家兔急性肾功能衰竭的实验研究

Experimentalstudyoftheprotectiveeffectsofastragalusandsalviamiltiorrhizabungeonglycerolin┐ducedacuterenalfailureinrabbits

为寻找防治急性肾功能衰竭的合理用药，作者设计了用黄芪、丹参浸剂防治急性肾功能衰竭（ＡＲＦ）的实验研究。实验动物分黄芪（ＡＢ）组、丹参（ＳＭ）组、黄芪和丹参混合（ＡＢＳＭ）组及生理盐水（Ｃ）组，Ｃ组作对照性观察。以肌注甘油制成实验模型后，各组均作定期的各项肾功能生化值的测定及肾组织光、电形态学病理检查。实验结果表明：甘油致家兔ＡＲＦ实验模型，其损害不仅限于肾小管各段，而且损伤肾小球的超微结构，致使肾小球毛细血管功能丧失，肾小球滤过功能严重受损，成为尿闭的根源。两种浸剂对ＡＲＦ的防治效果对比，黄芪优于丹参（Ｐ＜０．０１）。提示黄芪是防治ＡＲＦ比较理想的药物。

Theastragalusandsalviamiltiorrhizabungealcoholextractswereusedinpreventivetreatmentofglycerolinducedacuterenalfailure(ARF)inrabbits.Theexperimentalrabbitsweredivid-ed:astragalusgroup(AB),salviamiltiorrhiabungegroup(SM),twoextractsmixturegroup(ABSM),andnormolsalinecontrolgroup(C).Theextracts,mixturesandnormolsalinewereadmin-istratedbeforeandaftertheinducedARF.ThecountsofNa,K,creatinineinurineandbloodanduri-naryAAP,NAGweredeterminatedduring24～14daysperiodicalyinal4groups,andtherenaltissuesweretakenfromsameperiodsforpathomorphologicalstudiesbymicroscopyandtransmissionelectromi-croscopy.ThedamageoftheglycerolinducedARFwasnotonlyintheconvolutetubulesbutalsointheglomerulus.Theglomerulorfiltrationratereducedabruptly,andoligouriaorauriadeveloped.ThestudyofrenalfunctionsandrenalmorphogyshowedthattheABandABSMgroupsweredamagedmorelesserandpromptelyrepairedthantheSMandCgroupsintheearlystage.NoglomerularsclerosiswasnotedintheAB,ABSMgroupsinlaterstage,butitoccuredsporadicalyordiffuselyintheSM,Cgroups.Therefore,astragalusisanidealprotectivedrugoftraditionalchinesemedicineforARF.