3个不同群体罗氏沼虾线粒体16SrRNA基因序列分析及遗传差异

Sequence Analysis and Genetic Diversity of the Mitochondrial 16SrRNA in Three Populations of Sprawn(Macrobrachium rosenbergii)

利用PCR技术扩增获得罗氏沼虾线粒体16SrRNA基因的部分片段,通过序列测定和用限制性内切酶MboI、AluI和TaqI分别对上述PCR产物进行限制性片段长度多态分析(RFLP),分析了取自缅甸引进种子代、广西养殖群体及江苏养殖群体3个不同群体罗氏沼虾的遗传差异。结果表明:在3个群体间序列同源性对比无变异,PCR-RFLP未发现有片段多态性,3个群体间不存在遗传结构差异。可能表明这3个群体的罗氏沼虾起源同一个种群,且分化较低。

The genetic differentia among 3 populations of sprawn （ Macrobrachium rosenbergii） collected from Guangxi province （GXM, YZM） and Jiangsu province （JSM） were examined with mitochondrial DNA sequence. About 426 base pairs of the 16S ribosomal RNA （16SrRNA） gene from 30 individuals （ 10 samples of each population） were amplified by polymerase chain reaction （PCR）. One of PCR products of each population was sequenced respectively, and base substitution was not found among 3 populations. In restriction fragment length polymorphism （RFLP）, three endonucleases （MboI, AluI, TaqI） recognized 6 - bp were used to digest the PCR products, none of them revealed polymorphic fragments. These results indicate that the genetic differentia among 3 populations is significantly low, and they may be origin of the same wild stock.