镧-水溶对氮蒽蓝光度法测定庆大霉素及妥布霉素

La(Ⅲ)and water-soluble aniline blue speetrophotometric determination of gentamycin and tobramycin

In acid solution,La（Ⅲ）and water-soluble aniline blue（ABWS）reacted with gentamycin sulfate（GEN）and tobramycin sulfate（TOB）respeetively,to form ternary ion association complexes wilh an obvious absorption peak and an fading peak,and cause the fading of ABWS.The maximum positive absorption wavelengths were 672nm,and their molar absorptivities（Ε）were 1.66×104L·mol·cm-1（GEN）and 2.59×104L·mol-1·cm-1（TOB）The maximum negative absorption wavelengths arere 610nm（GEN）and 614nm（TOB）,and their molar absorptivities（ε）arere 4.78×104L·cm-1（GEN）and 7.45×104L·mol-1·cm-1（TOB）;When the dual-wavelength absorption spectrometry was used to determine the concentrations of gentamycin or tobramycin,their molar absorptivities ε can reach 6.44×104L·mol-1·cm-1（GEN）and 1.00×105L·mol-1·cm-1（TOB）.This method was used to determine the content of gentamycin and tobramycin in inject lipuid and in human urine with satisfactory results.

In acid solution, La（ Ⅲ ）and water- soluble aniline blue（ABWS） reacted with gentamyein sulfate（GEN） and tobramycin sulfate（TOB）respeetively,to form ternary ion association complexes wilh an obvious absorption peak and an fading peak,and cause the fading of ABWS. The maximum positive absorption wavelengths were 672nm, and their molar absorptivities （E） were 1.66 × 10^4 L · mol · cm^-1 （GEN） and 2. 59 × 10^4L · mol^-1 · cm^-1 （TOB） The maximum negative absorption wavelengths arere 610nm （GEN） and 614nm （TOB）, and their molar absorptivities （ε） arere 4. 78 × 10^4 L · cm^- 1 （GEN） and 7. 45 × 10^4 L · mol^-1 · cm^- 1 （TOB） ; When the dual - wavelength absorption spectrometry was used to determine the concentrations of gentamyein or tobramycin, their molar absorptivities e can reach 6. 44 × 10^4L · mol^-1 · cm^-1 （GEN） and 1.00 × 10^5L · mol^-1 · cm^-1 （TOB）. This method was used to determine the content of gentamycin and tobramycin in inject lipuid and in human urine with satisfactory resuhs.