摘要
Commercial Isomaltooligosaccharide was purified by glucose oxidase(GOD)and catalase(CAT).Temperature,pH value,sugar concentration and enzyme concentration were determined by uniform design and single factor experiment.The optimal condition was that:temperature was 35-40℃,pH value was about 4.0,sugar concentration was 200g/L,reaction time was 24h,GOD concenation was 100U/g sugar,the best ratio of GOD to CAT was 15.Under the optinal condition,the isomalto-oligosaccharide with a purity of 85.28% was obtained.
Commercial Isomaltooligosaccharide was purified by glucose oxidase （GOD） and catalase （ CAT ）. Temperature, pH value, sugar concentration and enzyme concentration were determined by uniform design and single factor experiment. The optimal condition was that： temperature was 35 - 40℃, pH value was about 4. 0, sugar concentration was 200g/L, reaction time was 24h, GOD concenation was 100U/g sugar,the best ratio of GOD to CAT was 15. Under the optinal condition, the isomalto -oligosaccharide with a purity of 85. 28% was obtained.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2005年第5期674-676,共3页
Chemical Research and Application
关键词
复合酶
提纯
异麦芽低聚糖
combinedenzyme
purify
isomalto - oligosacchafide
