微波辐射对几种常见表皮癣菌杀灭或抑制效应的实验研究

An experimental study of the inhibiting and killing effects of microwave diathermy on some epidermophytosis fungi

目的探讨微波辐射对红色毛癣菌、犬小孢子菌、须癣毛癣菌杀抑的效应。方法采用ME7250型、频率2450MHz的医用微波仪,分别以输出功率为20,40,60W的微波,辐射接种后的红色毛癣菌、犬小孢子菌、须癣毛癣菌,并对其生长情况进行实验室观察。将接种的红色毛癣菌、犬小孢子菌、须癣毛癣菌的试管,分别按不同的辐射时间、辐射次数分为辐射10,15,20min三大组;每一辐射时间档中分为辐射1,3,6,9次的组别。按设计分组辐射完成后,置27℃恒温箱内。30d后,观察试管内菌落的生长情况,并在显微镜下对其生物学形态进行观察。结果输出功率20W的微波辐射无明显的杀抑效应。输出功率40W的微波辐射10min,15min有抑菌的效应;辐射20min有较明显抑菌的效应。输出功率60W的微波辐射10min,有较明显抑菌的效应,辐射15min,20min显现杀菌效应。结论输出功率40W的微波辐射有抑菌的效应。输出功率60W、频率2450MHz的微波辐射红色毛癣菌、犬小孢子菌、须癣毛癣菌,有明显的抑制生长和杀菌作用,效应随辐射强度的增大而增强。

Objective To study the inhibiting and killing effects of microwave diathermy on some epidermophytosis fungi. Methods The cultured T. rubrum, M. canis and T. mentagrophytes were irradiated with a therapeutic microwave diathermy apparatus with the frequency of 2 450 MHz and power of 20 W, 40 W and 60 W, respectively. Each fungus was divided into different groups according to the duration of irradiation each time （ eg. 10, 15 and 20 minutes）, and times of irradiation （eg. 1, 3, 6 and 9 times）. All the samples of the fungi were cultured at 27℃ for 30 days after radiation and then observed under microscope. Results It was shown that 20 W of microwave had no inhibiting or killing effect on the various fungi studied ; 10, 15 and 20 minutes of irradiation with 40 W microwave could inhibit the growth of the various fungi; 15 and 20 minutes of irradiation with 40 W microwave had significant killing effect on the various fungi. Conclusion Microwave of 2 450 MHz and 40 W had significant inhibiting effects while 60 W microwave had significant killing effects on the T. rubrum, M. canis and T. mentagrophytes in the condition of the experiment. The inhibiting and killing effect was dose-dependent on the microwave irradiation.