摘要
利用分子梳方法结合荧光显微术对单个DNA-YOYO-1复合体的光漂白过程进行了研究,发现单个DNA-YOYO-1复合体的光漂白是一个随光照时间指数衰减的过程,并给出了DNA拓扑结构以及光照强度对光漂白过程的影响.
The two dyes named YOYO and TOTO used as fluorescent probes in DNA experiments were reported in 1992. When the cyanine dye YOYO is bound to DNA its fluorescence quantum yield can be 1000-fold . higher than when it is free in solution . Because of this YOYO dye is widely used not only for detection of DNA in electrophoresis and visualization of DNA in fluorescence microscopy, but also for single molecular detection of DNA. On the other hand,
基金
ProjectsupportedbytheNationalNaturalScienceFoundationofChina(60025516,10334100).
关键词
DNA
YOYO
光漂白
分子梳
DNA, YOYO, Photobleaching, Molecular combing
