内皮抑素基因联合重组干扰素-α蛋白对卵巢癌抑制作用的研究

Study on Inhibitory Effect of Endostatin Gene Combined with Recombinant IFN-α Protein on Ovarian Cancer

目的:探讨联合应用内皮抑素基因和重组干扰素-α(IFN-α)蛋白对鼠卵巢癌移植瘤的抑制作用。方法:利用人卵巢上皮癌细胞株(3AO细胞)制备卵巢癌移植瘤动物模型,以重组IFN-α和脂质体介导分泌型内皮抑素重组载体基因(pVAX-sEn)瘤内及瘤周注射,每周2次,测量瘤体大小,流式细胞仪检测pVAX-sEn基因转染对肿瘤细胞细胞周期的影响,免疫组化法检测肿瘤微血管密度。结果:联合注射组瘤体体积显著小于pVAX-sEn单用组(P<0.01);联合治疗组肿瘤细胞凋亡率为18.5%,高于pVAX-sEn单用组;联合治疗组肿瘤细胞坏死明显,血管较少,肿瘤微血管密度为3.16±1.12,明显少于pVAX-sEn单用组(P<0.01)。结论:脂质体介导pVAX-sEn瘤内注射可有效抑制卵巢癌移植瘤的生长,联合应用重组IFN-α效果更显著。

Objective： To study inhibitory effect of endcetatin gene combined with recombinent IFN - α protein on transplantation tumor of ovarian cancer. Methods： 3AO cells line were used to prepare ovarian cancer animal model, recombinant IFN -α combined with lipofectin mediated pVAX-sEn were injected intra-tumor and peri-tumor, the volumes of tumor were measured two times one week; Flow Cytometry and histoimmnochemical assay was used to detect the effect of pVAX-sEn on tumor cell cycle and microvessels density of tumor tissue, respectively. Results： The tumor volumes in the combined treatment group were significantly smaller than those in pVAX-sEn treatment group （ P 〈 0.01）; The apoptosis rate of tumor cells in combined treatment group was 18.5%, which was higher than that in pVAX-sEn treatment group; There were obviously necrosis of tumor cells with fewer blood vessels in combined treatment group. The microvessels density in combined treatment group was 3.16 ± 1.12, which was significantly lower than pVAX-sEn treatment group （P〈0.01）. Conclusions： Upofectin mediated pVAX-sEn gene expressing intra-tumor can effectively inhibit the growth of transplantation tumor of ovarian cancer, the inhibitory effect is better when combined with recombinant IFN-α.