血红素氧化酶-1、2及内皮素-1在妊娠高血压综合征患者胎盘组织中的表达及作用

Expression of Heme Oxygenase-1?2 and Endothelin-1 in Human Placental Villus and Hheir Effect on Pregnancy-induced Hypertension

目的:通过观察妊娠高血压综合征(简称妊高征,PIH)患者胎盘组织血红素氧化酶-1、2(HO-1、2)及内皮素-1(ET-1)的表达,探讨PIH发病机制。方法:采用免疫组化SABC法,检测20例PIH患者及正常孕妇的胎盘绒毛组织中的HO-1、2及ET-1,利用免疫组化图象分析软件测量胎盘大绒毛、微绒毛合体滋养细胞及血管的平均吸光度。结果:妊高征组大绒毛、微绒毛合体滋养细胞及血管部位的HO-2表达显著低于正常孕妇组(P<0.001),HO-1在胎盘合体滋养细胞的表达显著低于正常孕妇组(P<0.001),而妊高征组大绒毛、微绒毛合体滋养细胞及血管部位的ET-1表达显著高于正常孕妇组(P<0.001);且H0-1、2在胎盘大绒毛、微绒毛合体滋养细胞的表达与平均动脉压呈负相关,而ET-1在胎盘大绒毛、微绒毛合体滋养细胞的表达与平均动脉压呈正相关。结论:妊高征患者胎盘组织血红素氧化酶的表达减少可能在妊高征的发生、发展过程中起着重要的作用。

Objective： To study the expression of herne oxygenase-1 （ HO- 1 ） 、 heme oxygenase-2 （HO-2） and endothelin - 1 （ ET- 1 ） in the placental villus of pregnancy-induced hypertension （PIH） and their effect on pathogenesis of PIH. Methods： Immunohistochemistry was used to examine the expression of the HO-1 、HO-2 and ET-1 in the placental villus tissues of 20 PIH patients and 20 women with normal term pregnancy （NTP）. Immunohistochemistry image analysis systems was used to assess the average value of integral light density（LD） in the blood vessel and syncytiotrophoblast（STB） cells of the villus and microvillus as relative content of HO-1 、HO-2 and ET-1. Results： Expression of HO-2 in the STB cells and blood vessel of villus and mirovillus was significantly lower in PIH group than in NTP group （ P 〈 0.001 ）, but expression of ET-1 in these location was higher in PIH group than in NTP group （ P 〈 0.001 ）. Expression of HO-1 in the STB cells was higher in NTP group than in PIH group （ P 〈 0.001 ）. The pregnant women＇ s mean artery pressure （MAP） was inversely related to the relative content （LD） of HO-1 and HO- 2, but positively related to the relative content （LD） of ET-1 in the STB cells of placental villus and microvillus. Conclusions： The decrease of HO and increase of ET-1 in the placenta of PIH patients may be one of the important mechanisms for the onset of PIH.