中国地方黄牛GH基因遗传多态性研究

Genetic Polymorphisms of GH Genes in Chinese Yellow Cattle

以鲁西牛、南阳牛为试验动物,利用PCR-SSCP和PCR-RFLP技术研究了以上2个地方黄牛品种生长激素(GH)基因第3内含子、第4内含子、第5外显子、3′端和5′端位点的遗传多态性。检测结果表明:GH基因第3内含子1 547 bp处发生碱基C→T的替换;GH基因第4内含子位点多态的产生是由于1 947 bp处发生碱基T→G的替换;GH基因第5外显子2 141 bp处碱基C→G的突变,使得亮氨酸变成缬氨酸,造成氨基酸发生变化。GH基因3′端的PCR-RFLP结果表明:由于2 639 bp处碱基GCC→GGC的突变造成HaeⅢ酶增加1个酶切位点;GH基因5′端位点多态的产生是由303 bp处碱基C→T的突变造成。

Nanyang and Luxi cattle were used as experimental population; the genetic variations of GH genes were researched by PCR-SSCP and PCR-RFLP method. The results were as follows. The polymorphic PCR-SSCP sites in the 3^rd , 4^th intron, the 5^th exon, and 5＇ flanking region and the polymorphic HaeⅢ site in the 3＇ flanking region of the bovine growth hormone （GH） were examined in two breeds. The polymorphisms of the 3^rd intron were caused by C→T transition at 1 547 bp position. The polymorphisms of the 4^th intron were tested by T→G transition at 1 947 bp position in Nanyang, Luxi cattle. The polymorphisms of the 5^th exon were caused by C→G transition at 2 141 bp position, which resulted amino acid translation, leucine to valine. And the polymorphic HaeⅢ site in the 3＇ flanking region were caused by GCC→GGC transition at 2 639 bp position, and C→T transition at 303 bp resulted the polymorphisms of 5＇ flanking region.