摘要
将400只1日龄AA肉仔鸡随机分成4个处理,每个处理5个重复,每个重复20只鸡(公母各半);基础日粮为小麦-酪蛋白型日粮,在基础日粮中分别添加0.30、0.90和2.7 mg/kg 3个水平的生物素,包括对照组共4种日粮处理。试验结果表明:(1)添加生物素可促进免疫器官的发育,提高其质量指数;提高了血清中新城疫抗体滴度和IgG水平,0.9 mg/kg和2.7 mg/kg处理组的新城疫抗体滴度显著高于对照组和0.3 mg/kg处理组(P<0.05),2.7 mg/kg处理组的IgG水平显著高于对照组(P<0.05);(2)添加生物素显著提高了血液中B淋巴细胞的转化效率(P<0.05)和脾脏中T淋巴细胞(P<0.05)和B淋巴细胞的转化效率(P<0.05);显著降低了血液中表达CD3+的细胞含量(P<0.05),其中2.7 mg/kg处理组极显著低于对照组和0.3 mg/kg处理组(P<0.05);显著降低了脾脏中表达CD3+、CD4+和CD8+的细胞含量(P<0.05),其中2.7 mg/kg处理组显著低于对照组和0.3 mg/kg处理组(P<0.05);(3)添加生物素后,血清甲状腺素T4、三碘甲状原氨酸T3、生长激素和皮质醇水平呈上升趋势(P>0.05)。
400 AA broilers of 1 day were allocated into 4 treatments, 5 replicates per treatment, 20 broilers per replicate. The basal diet was wheat-casein diet. Biotin was added in the basal diet at 0.3 mg/kg, 0.9 mg/kg and 2.7 mg/kg. There were 4 diets including the control of basal diet. The results showed as follows. (1) Addition of biotin enhanced their development and elevated their weight index. The antibody titer to Newcastle and IgG content were elevated by addition of biotin. The antibody titer to Newcastle of 0.9 mg/kg and 2.7 mg/kg treatments were higher significantly than that of the control and 0.3 mg/kg treatment (P〈0.05). The IgG level of 2.7 mg/kg treatment was higher markedly than that of the control (P〈0.05). (2) Addition of biotin enhanced significantly B-lymphocyte transformation ratio in blood (P〈0.05), and T-lymphocyte and B-lymphocyte (P〈0.05) transformation ratio in spleen. In spleen, the cell content of expressing CD3^+, CD4^+ and CD8^+ were reduced significantly by addition of biotin (P〈0.05). The cell content of expressing CD3^+, CD4^+ and CD8^+ of 2.7 mg/kg treatment was lower markedly than that of the control and 0.3 mg/kg treatment (P〈0.05). In blood, addition of biotin reduced significantly the cell content of expressing CD3^+ (P〈0.05). The cell content of expressing CD3^+ of 2.7 mg/kg treatment was lower markedly than that of the control and 0.3 mg/kg treatment (P〈0.05). (3) Biotin addition elevated serum T3, T4, growth hormone and cortisol (P〉0.05), and what's more, no noted difference existed among the biotin addition treatments (P〉0.05).
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第10期1006-1013,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA